PMID- 15813943
OWN - NLM
STAT- MEDLINE
DCOM- 20050527
LR  - 20141120
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 21
IP  - 5
DP  - 2005 Mar
TI  - Serotonin refines the locomotor-related alternations in the in vitro neonatal rat 
      spinal cord.
PG  - 1338-46
AB  - Serotonergic projections from raphe nuclei arrive in the lumbar enlargement of 
      the spinal cord during the late fetal period in the rat, a time window during 
      which the locomotor-related left/right and flexor/extensor coordinations switch 
      from synchrony to alternation. The goal of the present study was to investigate 
      the role played by serotonin (5-HT) in modulating the left/right and 
      flexor/extensor alternations. Fictive locomotion was induced by bath application 
      of N-methyl-D,L-aspartate (NMA) in the in vitro neonatal rat spinal cord 
      preparation. By means of cross-correlation analysis we demonstrate that 5-HT, 
      when added to NMA, improves left/right and flexor/extensor (recorded from the 3rd 
      and 5th lumbar ventral roots, respectively) alternations. This effect was partly 
      reproduced by activation of 5-HT(2A/2C) receptors. We then tested the 
      contribution of endogenous 5-HT to NMA-induced fictive locomotion. Reducing the 
      functional importance of endogenous 5-HT, either by inhibiting its synthesis with 
      daily injections of p-chloro-phenylalanine (PCPA), starting on the day of birth, 
      or by application of ketanserin (a 5-HT(2) receptor antagonist) or SB269970 (a 
      5-HT(7) receptor antagonist), disorganized the NMA-induced locomotor pattern. 
      This pattern was restored in PCPA-treated animals by adding 5-HT to the bath. 
      Blocking 5-HT(7) receptors disorganized the locomotor-like rhythm even in the 
      absence of electrical activity in the brain stem, suggesting that NMA applied to 
      the spinal cord does not cause 5-HT release by activating a spino-raphe-spinal 
      loop. These results demonstrate that 5-HT is critical in improving the 
      locomotor-related alternations in the neonatal rat.
FAU - Pearlstein, E
AU  - Pearlstein E
AD  - Laboratoire Plasticite et Physio-Pathologie de la Motricite (PM), CNRS & 
      Universite de la Mediterranee, UMR 6196, CNRS, 31 Chemin Joseph Aiguier, F-13402 
      Marseille Cedex 20, France. pearlst@dpm.cnrs-mrs.fr
FAU - Ben Mabrouk, F
AU  - Ben Mabrouk F
FAU - Pflieger, J F
AU  - Pflieger JF
FAU - Vinay, L
AU  - Vinay L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Drug Combinations)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Phenols)
RN  - 0 (SB 269970)
RN  - 0 (Serotonin Antagonists)
RN  - 0 (Sulfonamides)
RN  - 333DO1RDJY (Serotonin)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 97F9DE4CT4 (Ketanserin)
RN  - R5J7E3L9SP (Fenclonine)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Drug Interactions
MH  - Electrophysiology
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Fenclonine/pharmacology
MH  - Functional Laterality
MH  - In Vitro Techniques
MH  - Ketanserin/pharmacology
MH  - Motor Activity/drug effects/*physiology
MH  - N-Methylaspartate/pharmacology
MH  - Phenols/pharmacology
MH  - Rats
MH  - Serotonin/*physiology
MH  - Serotonin Antagonists/pharmacology
MH  - Spinal Cord/drug effects/*physiology
MH  - Statistics as Topic
MH  - Sulfonamides/pharmacology
EDAT- 2005/04/09 09:00
MHDA- 2005/05/28 09:00
CRDT- 2005/04/09 09:00
PHST- 2005/04/09 09:00 [pubmed]
PHST- 2005/05/28 09:00 [medline]
PHST- 2005/04/09 09:00 [entrez]
AID - EJN3971 [pii]
AID - 10.1111/j.1460-9568.2005.03971.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2005 Mar;21(5):1338-46. doi: 10.1111/j.1460-9568.2005.03971.x.