PMID- 15814598
OWN - NLM
STAT- MEDLINE
DCOM- 20050711
LR  - 20151119
IS  - 0305-7453 (Print)
IS  - 0305-7453 (Linking)
VI  - 55
IP  - 5
DP  - 2005 May
TI  - Antibacterial activity of linezolid and vancomycin in an in vitro pharmacodynamic 
      model of gram-positive catheter-related bacteraemia.
PG  - 792-5
AB  - OBJECTIVES: The aim of this study was to compare the activity of linezolid and 
      vancomycin in an in vitro pharmacodynamic model to assess potential differences 
      in activity against biofilm-embedded organisms. METHODS: Single-lumen central 
      venous catheters colonized with biofilm-embedded Staphylococcus aureus, 
      Staphylococcus epidermidis or vancomycin-resistant Enterococcus faecium (VRE) 
      were treated with simulated clinical dosing regimens of linezolid 600 mg every 12 
      h or vancomycin 1 g every 12 h in a one-compartment in vitro pharmacodynamic 
      model. Quantitative cultures were sampled through the catheter and peripheral 
      ports over 48 h to dynamically assess changes in the burden of catheter 
      colonization and organism seeding, respectively. At 24 and 48 h catheters were 
      removed, sonicated and cultured for adherent organisms. RESULTS: Both linezolid 
      and vancomycin suppressed bacterial growth on the catheter and release of S. 
      aureus and S. epidermidis into the model compared with controls (P < 0.05), while 
      linezolid also suppressed counts compared with control and vancomycin versus VRE. 
      Neither agent completely eradicated bacterial colonization of the catheters. MICs 
      for the isolates recovered from the model did not increase over time with 
      linezolid or vancomycin exposure. CONCLUSIONS: Lack of activity against 
      biofilm-embedded organisms appeared to be the primary reason for microbiological 
      failure of both drugs in the model.
FAU - Wiederhold, Nathan P
AU  - Wiederhold NP
AD  - The University of Houston College of Pharmacy, Houston, TX 77030, USA.
FAU - Coyle, Elizabeth A
AU  - Coyle EA
FAU - Raad, Issam I
AU  - Raad II
FAU - Prince, Randall A
AU  - Prince RA
FAU - Lewis, Russell E
AU  - Lewis RE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050406
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - 0 (Acetamides)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Oxazolidinones)
RN  - 6Q205EH1VU (Vancomycin)
RN  - ISQ9I6J12J (Linezolid)
SB  - IM
MH  - Acetamides/*pharmacology
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacteremia/microbiology
MH  - Biofilms/*drug effects/growth & development
MH  - Catheterization, Central Venous/*adverse effects
MH  - Colony Count, Microbial
MH  - Enterococcus faecium/drug effects
MH  - Gram-Positive Bacterial Infections/microbiology
MH  - Gram-Positive Cocci/*drug effects
MH  - Humans
MH  - Linezolid
MH  - Microbial Sensitivity Tests
MH  - *Models, Biological
MH  - Oxazolidinones/*pharmacology
MH  - Staphylococcus aureus/drug effects
MH  - Staphylococcus epidermidis/drug effects
MH  - Vancomycin/pharmacokinetics/*pharmacology
EDAT- 2005/04/09 09:00
MHDA- 2005/07/12 09:00
CRDT- 2005/04/09 09:00
PHST- 2005/04/09 09:00 [pubmed]
PHST- 2005/07/12 09:00 [medline]
PHST- 2005/04/09 09:00 [entrez]
AID - dki106 [pii]
AID - 10.1093/jac/dki106 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2005 May;55(5):792-5. doi: 10.1093/jac/dki106. Epub 2005 
      Apr 6.