PMID- 15818616
OWN - NLM
STAT- MEDLINE
DCOM- 20050803
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 116
IP  - 3
DP  - 2005 Sep 1
TI  - hTERT gene copy number is not associated with hTERT RNA expression or telomerase 
      activity in colorectal cancer.
PG  - 395-400
AB  - In a majority of malignant human tumors telomerase activity can be detected, 
      suggesting an immortal phenotype. Expression of the reverse transcriptase 
      subunit, hTERT, in the human telomerase complex is required for telomerase 
      activity. The regulation of hTERT, from gene level to a fully functional protein, 
      is still a poorly understood process. Increased copy number of the hTERT gene has 
      been demonstrated in a significant portion of established cell lines and tumors 
      of different origin but its relevance for telomerase activity levels is unclear. 
      In the present study, we examined the hTERT gene copy number using fluorescence 
      in situ hybridization (FISH) in samples from 64 colorectal carcinomas and an 
      increased copy number (> or = 3 hTERT gene copies/nucleus) was observed in 31 
      cases (48%). No statistical association existed between hTERT gene copy number 
      and hTERT RNA expression or telomerase activity. However, a significant 
      relationship was found between an increase in hTERT gene copy number and p53 
      protein accumulation (p = 0.002) and aneuploidy (p = 0.036). Only 4 tumors showed 
      microsatellite instability, 3 of which had a normal hTERT gene copy number. The 
      data indicated that the increased copy number of the hTERT gene in colorectal 
      carcinoma was a result of genomic instability with no obvious consequence for 
      telomerase activity levels.
FAU - Palmqvist, Richard
AU  - Palmqvist R
AD  - Department of Medical Biosciences, Pathology, Umea University, Umea, Sweden.
FAU - Zhang, Anju
AU  - Zhang A
FAU - Xu, Dawei
AU  - Xu D
FAU - Golovleva, Irina
AU  - Golovleva I
FAU - Norrback, Karl-Fredrik
AU  - Norrback KF
FAU - Gruber, Astrid
AU  - Gruber A
FAU - Oberg, Ake
AU  - Oberg A
FAU - Stenling, Roger
AU  - Stenling R
FAU - Roos, Goran
AU  - Roos G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (DNA-Binding Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Aneuploidy
MH  - Colorectal Neoplasms/*enzymology/*genetics
MH  - DNA-Binding Proteins
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microsatellite Repeats
MH  - RNA/biosynthesis
MH  - Telomerase/*biosynthesis/*genetics/*physiology
EDAT- 2005/04/09 09:00
MHDA- 2005/08/04 09:00
CRDT- 2005/04/09 09:00
PHST- 2005/04/09 09:00 [pubmed]
PHST- 2005/08/04 09:00 [medline]
PHST- 2005/04/09 09:00 [entrez]
AID - 10.1002/ijc.21020 [doi]
PST - ppublish
SO  - Int J Cancer. 2005 Sep 1;116(3):395-400. doi: 10.1002/ijc.21020.