PMID- 15818735
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 15
DP  - 2005 Apr 21
TI  - In vitro growth inhibition of human colonic tumor cells by Verapamil.
PG  - 2255-9
AB  - AIM: To investigate the effects and mechanisms of Verapamil on cultured human 
      colonic tumor (HCT) cells. METHODS: HCT cells were treated with different 
      concentrations of Verapamil, and their proliferation was examined by MTT assay. 
      The areas of sub-diploid peak were measured by flow cytometry, and the DNA ladder 
      was found by agarose gel electrophoresis. The characteristic changes in 
      morphology were observed under light microscopy. The cell nuclei (propidium 
      iodide labeled, PI-labeled) and cellular distribution and concentration of 
      calcium (Fluo-3-labeled) were studied by using laser confocal scanning 
      microscope. RESULTS: The proliferation of HCT cells was inhibited by different 
      concentrations of Verapamil. With the increase in concentration of Verapamil, the 
      percent of G0-G1 phase cells in HCT cells increased and that of S phase cells 
      decreased. After treating with different concentrations of Verapamil, flow 
      cytometry showed that HCT cells were enlarged in areas of sub-diploid in a 
      dose-dependent manner. Gel electrophoresis results displayed a typical DNA 
      ladder. On staining with Wrights-Giemsa, the typical cellular apoptosis 
      morphologic changes were also observed. PI-labeled cell nuclei were found 
      markedly changed. In addition, we inspected that the 100 micromol/L Verapamil 
      could increase the intracellular calcium ion concentration [Ca(2+)](i) in HCT 
      cells. CONCLUSION: Verapamil can inhibit proliferation of HCT cells via inducing 
      cell apoptosis.
FAU - Cao, Qi-Zhen
AU  - Cao QZ
AD  - Department of Pharmacology, Peking University Health Science Center, Peking 
      University, Beijing 100083, China.
FAU - Niu, Gang
AU  - Niu G
FAU - Tan, Huan-Ran
AU  - Tan HR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Calcium Channel Blockers)
RN  - CJ0O37KU29 (Verapamil)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Calcium Channel Blockers/*pharmacology
MH  - Cell Division/drug effects
MH  - Cell Line, Tumor
MH  - Colonic Neoplasms/*drug therapy/*pathology
MH  - Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - Verapamil/*pharmacology
PMC - PMC4305808
EDAT- 2005/04/09 09:00
MHDA- 2005/05/25 09:00
PMCR- 2005/04/21
CRDT- 2005/04/09 09:00
PHST- 2005/04/09 09:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2005/04/09 09:00 [entrez]
PHST- 2005/04/21 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i15.2255 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 Apr 21;11(15):2255-9. doi: 10.3748/wjg.v11.i15.2255.