PMID- 15820259
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20061115
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 124
IP  - 2
DP  - 2005 Apr
TI  - A technique of bone marrow collection from vertebral bodies of cynomolgus 
      macaques for transplant studies.
PG  - 280-8
AB  - BACKGROUND: Strategies to induce donor-specific allograft tolerance are best 
      tested in preclinical models developed in nonhuman primates (NHPs). Most 
      protocols prepare the recipient by infusing hematopoietic cells from the donor. 
      We report here a procedure to isolate and characterize large numbers of bone 
      marrow cells (BMCs) from cynomolgus monkeys (cynos) that can then successfully be 
      transplanted into conditioned recipients. MATERIALS AND METHODS: Vertebral 
      columns of five cynos were excised en bloc and separated into individual 
      vertebrae. The cancelous bone was extracted with a core puncher, fractionated, 
      filtered, centrifuged, and resuspended in transplantation media before being 
      analyzed by flow cytometry. In two instances, the collected BMCs were reinfused 
      into allogeneic recipients preconditioned with a nonmyeloablative regimen. 
      Chimerism was monitored using short-tandem repeat analysis. RESULTS: The mean 
      total BMCs yield was 25.5 x 10(9) (range of 4.00 x 10(9) to 59 x 10(9)) with mean 
      cell viability of 93.4% (range: 90-96%). CD34+ cells and CD3+ cells averaged 0.34 
      and 3.91% of total BMCs, respectively. This resulted in absolute cell number 
      yields of 1.02 x 10(8) and 1.15 x 10(9) for CD34+ and CD3+ cells, respectively. 
      Graft-versus-host disease was absent in both bone marrow infused animals, and a 
      maximum level of chimerism of 18% was detected at 3 weeks after BMCs infusion. 
      CONCLUSION: We present here the first detailed report of a procedure to retrieve 
      and characterize large numbers of BMCs from vertebral bodies of cynos and 
      demonstrate that cells collected with this technique have the capability of 
      engrafting in allogenic recipients.
FAU - Flores, Mona G
AU  - Flores MG
AD  - Transplantation Immunology Laboratory, Department of Cardiothoracic Surgery, Falk 
      Cardiovascular Research Center, Stanford University School of Medicine, Stanford, 
      CA 94305-5407, USA.
FAU - Holm, Bari
AU  - Holm B
FAU - Larson, Michael J
AU  - Larson MJ
FAU - Lau, Macy K
AU  - Lau MK
FAU - Si, Ming-Sing
AU  - Si MS
FAU - Lowsky, Robert
AU  - Lowsky R
FAU - Rousvoal, Geraldine
AU  - Rousvoal G
FAU - Grumet, F Carl
AU  - Grumet FC
FAU - Strober, Samuel
AU  - Strober S
FAU - Hoppe, Richard
AU  - Hoppe R
FAU - Reitz, Bruce A
AU  - Reitz BA
FAU - Borie, Dominic C
AU  - Borie DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Immunosuppressive Agents)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/immunology
MH  - Cell Separation/*methods
MH  - Graft Rejection/drug therapy
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*cytology/immunology
MH  - Immune Tolerance
MH  - Immunosuppressive Agents/pharmacology
MH  - Macaca fascicularis
MH  - Male
MH  - Spine/*cytology
MH  - Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2005/04/12 09:00
MHDA- 2005/05/25 09:00
CRDT- 2005/04/12 09:00
PHST- 2004/08/05 00:00 [received]
PHST- 2005/04/12 09:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2005/04/12 09:00 [entrez]
AID - S0022-4804(04)00615-8 [pii]
AID - 10.1016/j.jss.2004.09.018 [doi]
PST - ppublish
SO  - J Surg Res. 2005 Apr;124(2):280-8. doi: 10.1016/j.jss.2004.09.018.