PMID- 15820504
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20131121
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 76
IP  - 25
DP  - 2005 May 6
TI  - A novel L-glutamate transporter inhibitor reveals endogenous D-aspartate 
      homeostasis in rat pheochromocytoma MPT1 cells.
PG  - 2933-44
AB  - We previously reported for the first time that D-aspartate (D-Asp) is 
      biosynthesized by cultured mammalian cells such as pheochromocytoma (PC)12 cells 
      and its subclone MPT1 (FEBS Lett. 434 (1998) 231, Arch. Biochem. Biophys. 404 
      (2002) 92). We speculated that D-Asp levels in the intra- and extracellular 
      spaces of the cultured cells are maintained in a dynamic state of homeostasis. To 
      test this here, we utilized a novel and potent L-Glu transporter inhibitor, 
      TFB-TBOA. This inhibitor proved to be a genuine nontransportable blocker of the 
      transporter even during long periods of culture. Use of this inhibitor with MPT1 
      cells confirmed that D-Asp levels are in a dynamic steady state where it is 
      constantly released into the extracellular space by a yet undefined mechanism as 
      well as being constantly and intensively taken up by the cells via the L-Glu 
      transporter. We estimated the rate with which D-Asp is constitutively released 
      from MPT1 cells is approx. 3.8 pmol/h/1x10(5) cells.
FAU - Koyama, Hayato
AU  - Koyama H
AD  - School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, 
      Minato-ku, Tokyo 108-8641, Japan.
FAU - Sekine, Masae
AU  - Sekine M
FAU - Furuchi, Takemitsu
AU  - Furuchi T
FAU - Katane, Masumi
AU  - Katane M
FAU - Nimura, Noriyuki
AU  - Nimura N
FAU - Shimamoto, Keiko
AU  - Shimamoto K
FAU - Nakajima, Terumi
AU  - Nakajima T
FAU - Homma, Hiroshi
AU  - Homma H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (benzyloxyaspartate)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
SB  - IM
MH  - Amino Acid Transport System X-AG/*antagonists & inhibitors
MH  - Animals
MH  - Aspartic Acid/metabolism/*pharmacology
MH  - Biological Transport/drug effects/physiology
MH  - Chromatography, High Pressure Liquid
MH  - D-Aspartic Acid/*metabolism/physiology
MH  - Fluorescence
MH  - Homeostasis/*drug effects/physiology
MH  - PC12 Cells
MH  - Rats
EDAT- 2005/04/12 09:00
MHDA- 2005/06/09 09:00
CRDT- 2005/04/12 09:00
PHST- 2004/07/09 00:00 [received]
PHST- 2004/10/26 00:00 [accepted]
PHST- 2005/04/12 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/04/12 09:00 [entrez]
AID - S0024-3205(05)00086-X [pii]
AID - 10.1016/j.lfs.2004.10.057 [doi]
PST - ppublish
SO  - Life Sci. 2005 May 6;76(25):2933-44. doi: 10.1016/j.lfs.2004.10.057.