PMID- 1582265
OWN - NLM
STAT- MEDLINE
DCOM- 19920618
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 60
IP  - 1
DP  - 1992
TI  - Characterization of 10 marker chromosomes in a prostatic cancer cell line by in 
      situ hybridization.
PG  - 8-11
AB  - Marker chromosomes contain potentially valuable information about breakpoints in 
      cancer. However, routine banding procedures, by themselves, provide only limited 
      information about the identity of marker chromosomes. In this study, the use of 
      fluorescence in situ hybridization (FISH) with chromosome-specific centromeric 
      probes and whole-chromosome-specific DNA libraries greatly enhanced the 
      identification of 10 marker chromosomes in the primary prostatic cancer cell line 
      PPC-1. Centromeric probes for chromosomes 1, 2, 3, 4, 10, 12, and 17 and 
      whole-chromosome paint libraries for chromosomes 1, 2, 3, 4, 8, and 12, in 
      conjunction with analysis of G-banded metaphases, allowed the major portion(s) of 
      these 10 PPC-1 marker chromosomes to be defined. The results increase the number 
      of identifiable chromosomal breakpoints in this cell line from 9 to 28 sites.
FAU - Brothman, A R
AU  - Brothman AR
AD  - Department of Pediatrics, Eastern Virginia Medical School, Norfolk.
FAU - Patel, A M
AU  - Patel AM
LA  - eng
GR  - R01-CA46269/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Centromere
MH  - *Chromosomes, Human
MH  - *DNA Probes
MH  - Fluorescence
MH  - Gene Library
MH  - *Genetic Markers
MH  - Humans
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - Prostatic Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1159/000133283 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1992;60(1):8-11. doi: 10.1159/000133283.