PMID- 15822858
OWN - NLM
STAT- MEDLINE
DCOM- 20050712
LR  - 20131121
IS  - 0047-1917 (Print)
IS  - 0047-1917 (Linking)
VI  - 52
IP  - 4
DP  - 2005 Feb
TI  - Antigenotoxic effect of Pleurotus cornucopiae extracts on the mutagenesis of 
      Salmonella typhimurium TA98 elicited by benzo[a]pyrene and oxidative DNA lesions 
      in V79 hamster lung cells.
PG  - 163-72
AB  - Pleurotus cornucopiae (PC) mushroom with a brilliant yellow pileus is found in 
      the field and known in Japan as Tamogi dake mushroom. The purpose of this paper 
      is to investigate the mechanism of the antimutagenic effect of PC mushroom using 
      both the Ames test and Comet assay. We have found a strong inhibitory effect of 
      both aqueous and organic PC extracts on the mutagenicity elicited by 
      benzo[a]pyrene (B[a]P). This inhibition was dose-dependent in reaction mixtures 
      containing cytosolic and microsomal fractions (S-9) from untreated rat liver as 
      well as in those containing S-9 from aryl hydrocarbon receptor (Ah) ligand of 
      Sudan III-treated rats. Sudan III was a potent inducer of cytochrome P450 1A 
      (CYP1A) activity. We treated rats with Sudan III to enhance the metabolic 
      activation of B[a]P by the S-9 fraction. To explain whether this antimutagenicity 
      was due to the inhibition of CYP1A activity that metabolically activates B[a]P, 
      we tested the effects of the extracts on activities of CYP1A1 and CYP1A2, 
      represented by ethoxyresorufin O-deethylase (EROD) and methoxyresorufin 
      O-demethylase (MROD), respectively. Both aqueous and organic extracts inhibited 
      EROD activity at all dose levels, while the inhibitory effect was only observed 
      at high doses with regard to MROD activity. Furthermore, pre-treatment of Chinese 
      hamster V79cells with PC extracts significantly reduced H2O2-induced-DNA damage, 
      indicating that PC extracts provide a protective effect against oxidative DNA 
      damage. These results indicate that whole-mushroom extracts contain compounds 
      that may inhibit the metabolic activation of B[a]P by CYP1A1 as well as prevent 
      oxidative DNA damage.
FAU - El Bohi, Khlood M
AU  - El Bohi KM
AD  - Laboratory of Toxicology, Department of Environmental Veterinary Sciences, 
      Graduate School of Veterinary Medicine, Hokkaido University.
FAU - Sabik, Laila
AU  - Sabik L
FAU - Muzandu, Kaampwe
AU  - Muzandu K
FAU - Shaban, Zein
AU  - Shaban Z
FAU - Soliman, Mohamed
AU  - Soliman M
FAU - Ishizuka, Mayumi
AU  - Ishizuka M
FAU - Kazusaka, Akio
AU  - Kazusaka A
FAU - Fujita, Shoichi
AU  - Fujita S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Vet Res
JT  - The Japanese journal of veterinary research
JID - 0376567
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Biological Products)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - *Antimutagenic Agents
MH  - Benzo(a)pyrene/*antagonists & inhibitors/toxicity
MH  - Biological Products/pharmacology
MH  - Cell Line
MH  - Cricetinae
MH  - Cricetulus
MH  - *DNA Damage/drug effects
MH  - Hydrogen Peroxide/antagonists & inhibitors
MH  - Mutagenicity Tests
MH  - *Pleurotus
MH  - Salmonella typhimurium/drug effects/*genetics
EDAT- 2005/04/13 09:00
MHDA- 2005/07/13 09:00
CRDT- 2005/04/13 09:00
PHST- 2005/04/13 09:00 [pubmed]
PHST- 2005/07/13 09:00 [medline]
PHST- 2005/04/13 09:00 [entrez]
PST - ppublish
SO  - Jpn J Vet Res. 2005 Feb;52(4):163-72.