PMID- 15823398
OWN - NLM
STAT- MEDLINE
DCOM- 20050602
LR  - 20081121
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 62
IP  - 1
DP  - 2005 Jul
TI  - The use of RAPD genomic fingerprinting to study relatedness in strains of 
      Acidithiobacillus ferrooxidans.
PG  - 103-12
AB  - Twelve strains of Acidithiobacillus ferrooxidans were recovered from acid mine 
      drainage (AMD) sites from three different geographical locations: Copper Cliff, 
      Ontario, Canada; Mineral City, OH, USA; and Cornwall, England. The spread-plate 
      technique and various culture media were used to isolate and purify all strains. 
      DNA was extracted from each purified culture and amplified using PCR and twenty, 
      10-mer primers. Amplification products were separated by gel electrophoresis and 
      photographed under UV light. The RAPD (Randomly Amplified Polymorphic DNA) 
      profiles were compared on the basis of the presence or absence of each DNA band 
      and a data matrix was constructed. Strain diversity was calculated using the 
      Jaccard's coefficient and UPGMA (Unweighted Pair-Group Arithmetic Average 
      Clustering) cluster analysis. The variations in the banding patterns indicated 
      genomic variability among the twelve A. ferrooxidans strains tested. The primers 
      used in this study grouped the twelve strains into five major groups. 
      Similarities between the strains ranged from 5.49% to 85.14%. These results show 
      that the strains have a high degree of genomic diversity and that the RAPD 
      procedure is a powerful technique to assess strain variability in this bacterium.
FAU - Waltenbury, Danielle R
AU  - Waltenbury DR
AD  - Science Program, Science North, Sudbury, ON, Canada P3E 5S9.
FAU - Leduc, L G
AU  - Leduc LG
FAU - Ferroni, G D
AU  - Ferroni GD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (DNA, Bacterial)
RN  - 0 (Industrial Waste)
SB  - IM
MH  - Acidithiobacillus/classification/*genetics
MH  - Cluster Analysis
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/chemistry/genetics
MH  - Electrophoresis, Agar Gel
MH  - Genetic Variation
MH  - Industrial Waste
MH  - Mining
MH  - Random Amplified Polymorphic DNA Technique
EDAT- 2005/04/13 09:00
MHDA- 2005/06/03 09:00
CRDT- 2005/04/13 09:00
PHST- 2005/01/28 00:00 [received]
PHST- 2005/02/09 00:00 [revised]
PHST- 2005/02/09 00:00 [accepted]
PHST- 2005/04/13 09:00 [pubmed]
PHST- 2005/06/03 09:00 [medline]
PHST- 2005/04/13 09:00 [entrez]
AID - S0167-7012(05)00045-X [pii]
AID - 10.1016/j.mimet.2005.02.003 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2005 Jul;62(1):103-12. doi: 10.1016/j.mimet.2005.02.003.