PMID- 15823795
OWN - NLM
STAT- MEDLINE
DCOM- 20050616
LR  - 20191109
IS  - 1121-760X (Print)
IS  - 1121-760X (Linking)
VI  - 49
IP  - 1
DP  - 2005 Jan-Mar
TI  - High-level detection of gene amplification and chromosome aneuploidy in extracted 
      nuclei from paraffin-embedded tissue of human cancer using FISH: a new approach 
      for retrospective studies.
PG  - 53-8
AB  - A novel application of fluorescence in situ hybridization (FISH) to isolated 
      nuclei is described. The method detects gene amplification and chromosome 
      aneuploidy in extracted nuclei from paraffin-embedded tissue of human cancer with 
      greater sensitivity and specificity than existing FISH methods. In this study, 
      the method is applied to signal detection of the HER-2/neu (c-erbB-2) gene, whose 
      amplification is one of the most common genetic alterations associated with human 
      breast cancer. Nuclei were extracted and isolated from formalin fixed, paraffin 
      embedded tissue of 43 different carcinomas (breast, ovary, endometrium, 
      gastrointestinal stromal tumor and malignant mesothelioma). FISH was performed 
      both on sections and extracted nuclei of each tissue using chromosome enumeration 
      probes (CEP) for the centromeric regions of chromosomes 8 and 17, and a locus 
      specific identifier (LSI) for the HER-2/neu oncogene. Differences between ploidy 
      calculated in sections and extracted nuclei were seen in 3 breast carcinomas and 
      1 gastrointestinal stromal tumor (GIST). Furthermore, 1 breast cancer, previously 
      considered to be borderline for HER-2/neu gene amplification turned out to be 
      clearly amplified. Nuclei extraction and isolation bypass all the problems 
      related to signal interpretation in tissue sections, and the adoption of this new 
      technique, which improves the signal quality in several neoplastic samples, is 
      suggested.
FAU - Rossi, E
AU  - Rossi E
AD  - Department of Pathology, University of Brescia, Italy.
FAU - Ubiali, A
AU  - Ubiali A
FAU - Balzarini, P
AU  - Balzarini P
FAU - Cadei, M
AU  - Cadei M
FAU - Alpi, F
AU  - Alpi F
FAU - Grigolatoi, P G
AU  - Grigolatoi PG
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur J Histochem
JT  - European journal of histochemistry : EJH
JID - 9207930
SB  - IM
MH  - *Aneuploidy
MH  - Breast Neoplasms/genetics/pathology
MH  - Carcinoma/*genetics/pathology
MH  - Cell Nucleus/*genetics/pathology
MH  - Endometrial Neoplasms/genetics/pathology
MH  - Female
MH  - Gastrointestinal Neoplasms/genetics/pathology
MH  - Gene Amplification/*genetics
MH  - Genes, erbB-2/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mesothelioma/genetics/pathology
MH  - Neoplasms/*genetics/pathology
MH  - Ovarian Neoplasms/genetics/pathology
MH  - *Paraffin Embedding
MH  - Retrospective Studies
MH  - Sensitivity and Specificity
MH  - Tissue Fixation/methods
EDAT- 2005/04/13 09:00
MHDA- 2005/06/17 09:00
CRDT- 2005/04/13 09:00
PHST- 2005/04/13 09:00 [pubmed]
PHST- 2005/06/17 09:00 [medline]
PHST- 2005/04/13 09:00 [entrez]
AID - 10.4081/927 [doi]
PST - ppublish
SO  - Eur J Histochem. 2005 Jan-Mar;49(1):53-8. doi: 10.4081/927.