PMID- 15824323
OWN - NLM
STAT- MEDLINE
DCOM- 20050801
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 102
IP  - 18
DP  - 2005 May 3
TI  - Efficient cross-priming of tumor antigen-specific T cells by dendritic cells 
      sensitized with diverse anti-MICA opsonized tumor cells.
PG  - 6461-6
AB  - Dendritic cells (DCs) have the capacity to prime tumor-specific T cell responses 
      and are considered as potentially effective vaccines for immunotherapy of cancer. 
      Critical parameters in the development of DC vaccines are the source of tumor 
      antigen (TA) and the mode of DC-loading. Whole tumor cells contain complex 
      assortments of TA, which has been exploited to enhance cross-presentation to CD8 
      T cells by DCs loaded with anti-syndecan mAb-opsonized myeloma cells. This 
      approach may be broadly improved by targeting the MHC class I chain-related 
      protein A (MICA), which is frequently and abundantly expressed on most if not all 
      types of epithelial cancers but not in normal tissues except intestinal mucosa. 
      Loading of DC with anti-MICA mAb-coated breast, melanoma, or ovarian tumor lines 
      or uncultured ovarian cancer cells efficiently promoted TA cross-presentation and 
      priming of multivalent anti-tumor CD8 and CD4 T cell responses. These were of 
      substantially greater breadth and magnitude than those of T cells primed by 
      peptide-pulsed or apoptotic tumor cell-loaded DCs. These results may advance DC 
      vaccine development and provide a platform for adoptive T cell therapy and TA 
      discovery. These results further suggest that antibody targeting of MICA might be 
      applicable to elicit T cell immunity against tumors of diverse tissue origins in 
      cancer patients.
FAU - Groh, Veronika
AU  - Groh V
AD  - Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview 
      Avenue North, Seattle, WA 98109, USA. vgroh@fhcrc.org
FAU - Li, Yongqing Q
AU  - Li YQ
FAU - Cioca, Daniel
AU  - Cioca D
FAU - Hunder, Naomi N
AU  - Hunder NN
FAU - Wang, Wei
AU  - Wang W
FAU - Riddell, Stanley R
AU  - Riddell SR
FAU - Yee, Cassian
AU  - Yee C
FAU - Spies, Thomas
AU  - Spies T
LA  - eng
GR  - R37 AI030581/AI/NIAID NIH HHS/United States
GR  - R01 AI052319/AI/NIAID NIH HHS/United States
GR  - AI30581/AI/NIAID NIH HHS/United States
GR  - AI52319/AI/NIAID NIH HHS/United States
GR  - R01 AI030581/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050411
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA Primers)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (MHC class I-related chain A)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
CIN - Proc Natl Acad Sci U S A. 2005 May 3;102(18):6243-4. PMID: 15851655
MH  - Antibodies, Monoclonal/immunology/therapeutic use
MH  - Antigens, Neoplasm/*immunology
MH  - Cell Line, Tumor
MH  - Cross-Priming/*immunology
MH  - Cytotoxicity Tests, Immunologic
MH  - DNA Primers
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Immunity, Cellular/*immunology
MH  - Immunization, Passive/*methods
MH  - Interferon-gamma/metabolism
MH  - Neoplasms/immunology/*therapy
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/immunology
PMC - PMC1088382
EDAT- 2005/04/13 09:00
MHDA- 2005/08/02 09:00
PMCR- 2005/11/03
CRDT- 2005/04/13 09:00
PHST- 2005/04/13 09:00 [pubmed]
PHST- 2005/08/02 09:00 [medline]
PHST- 2005/04/13 09:00 [entrez]
PHST- 2005/11/03 00:00 [pmc-release]
AID - 0501953102 [pii]
AID - 01026461 [pii]
AID - 10.1073/pnas.0501953102 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2005 May 3;102(18):6461-6. doi: 
      10.1073/pnas.0501953102. Epub 2005 Apr 11.