PMID- 15828063
OWN - NLM
STAT- MEDLINE
DCOM- 20051216
LR  - 20191210
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 19
IP  - 9
DP  - 2005 Nov
TI  - Non-aqueous capillary electrophoresis for separation and simultaneous 
      determination of fraxin, esculin and esculetin in Cortex fraxini and its 
      medicinal preparations.
PG  - 696-702
AB  - A non-aqueous capillary electrophoresis method has been developed for the 
      separation and simultaneous determination of fraxin, esculin and esculetin in 
      Cortex fraxini and its preparation for the first time. Optimum separation of the 
      analytes was obtained on a 47 cm x 75 microm i.d. fused-silica capillary using a 
      non-aqueous buffer system of 60 mM sodium cholate, 20 mM ammonium acetate, 20% 
      acetonitrile and 3% acetic acid at 20 kV and 292 K, respectively. The relative 
      standard deviations (RSDs) of the migration times and the peak heights of the 
      three analytes were in the range of 0.23-0.28 and 2.12-2.60%, respectively. 
      Detection limits of fraxin, esculin and esculetin were 0.1557, 0.4073 and 0.5382 
      microg/mL, respectively. In the tested concentration range, good linear 
      relationships (correlation coefficients 0.9995 for fraxin, 0.9999 for esculin and 
      0.9992 for esculetin) between peak heights and concentrations of the analytes 
      were observed. This method has been successfully applied to simultaneous 
      determination of the three bioactive components with the recoveries from 90.2 to 
      109.2% in the five samples.
FAU - Li, Cunhong
AU  - Li C
AD  - Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of 
      China.
FAU - Chen, Anjia
AU  - Chen A
FAU - Chen, Xiaofeng
AU  - Chen X
FAU - Ma, Xiao
AU  - Ma X
FAU - Chen, Xingguo
AU  - Chen X
FAU - Hu, Zhide
AU  - Hu Z
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Acetates)
RN  - 0 (Acetonitriles)
RN  - 0 (Buffers)
RN  - 0 (Coumarins)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Solvents)
RN  - 0 (Umbelliferones)
RN  - 0 (qinpi)
RN  - 1Y1L18LQAF (Esculin)
RN  - NU3Y4CCH8Z (Sodium Cholate)
RN  - Q40Q9N063P (Acetic Acid)
RN  - RRE756S6Q2 (ammonium acetate)
RN  - SM2XD6V944 (esculetin)
RN  - V7M270Y072 (fraxin)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetates/chemistry
MH  - Acetic Acid/chemistry
MH  - Acetonitriles/chemistry
MH  - Buffers
MH  - Coumarins/*analysis/chemistry/isolation & purification
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Esculin/*analysis/chemistry/isolation & purification
MH  - Reproducibility of Results
MH  - Sodium Cholate/chemistry
MH  - Solvents/chemistry
MH  - Temperature
MH  - Umbelliferones/*analysis/chemistry/isolation & purification
EDAT- 2005/04/14 09:00
MHDA- 2005/12/17 09:00
CRDT- 2005/04/14 09:00
PHST- 2005/04/14 09:00 [pubmed]
PHST- 2005/12/17 09:00 [medline]
PHST- 2005/04/14 09:00 [entrez]
AID - 10.1002/bmc.497 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2005 Nov;19(9):696-702. doi: 10.1002/bmc.497.