PMID- 15829228
OWN - NLM
STAT- MEDLINE
DCOM- 20050630
LR  - 20191210
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1041
IP  - 2
DP  - 2005 Apr 18
TI  - Effect of hypoxanthine on Na+,K+-ATPase activity and some parameters of oxidative 
      stress in rat striatum.
PG  - 198-204
AB  - The main objective of this study was to investigate the effects of preincubation 
      of rat striatum homogenate in the presence of hypoxanthine, a metabolite 
      accumulated in Lesch-Nyhan disease, on Na+,K+-ATPase activity and on some 
      parameters of oxidative stress namely thiobarbituric acid-reactive substances 
      (TBA-RS), total radical-trapping antioxidant parameter (TRAP) and membrane 
      protein thiol content. Results showed that hypoxanthine significantly increased 
      TBA-RS and reduced Na+,K+-ATPase activity, TRAP and membrane protein thiol 
      content. In addition, we also evaluated the effect of glutathione, trolox, 
      allopurinol and Nvarpi-nitro-L-arginine methyl ester (L-NAME) on the inhibitory 
      effect of hypoxanthine on Na+,K+-ATPase activity in the same rat cerebral 
      structure. All tested compounds per se did not alter Na+,K+-ATPase activity, but 
      only glutathione and trolox prevented the effect of hypoxanthine on the enzyme 
      activity. The effect of glutathione and trolox on hypoxanthine-induced increase 
      of TBA-RS levels was also investigated. These antioxidants alone or combined with 
      hypoxanthine reduced TBA-RS levels. Our present findings show that hypoxanthine 
      induces oxidative stress in rat striatum and that the inhibition of Na+,K+-ATPase 
      activity caused by this oxypurine was probably mediated by reactive oxygen 
      species. It is presumed that these results might be associated with the neuronal 
      dysfunction of patients affected by Lesch-Nyhan disease.
FAU - Bavaresco, Caren Serra
AU  - Bavaresco CS
AD  - Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade 
      Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003 
      Porto Alegre, RS, Brazil.
FAU - Chiarani, Fabria
AU  - Chiarani F
FAU - Matte, Cristiane
AU  - Matte C
FAU - Wajner, Moacir
AU  - Wajner M
FAU - Netto, Carlos Alexandre
AU  - Netto CA
FAU - de Souza Wyse, Angela Terezinha
AU  - de Souza Wyse AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Antioxidants)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Free Radicals)
RN  - 0 (Membrane Proteins)
RN  - 0 (Sulfhydryl Compounds)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 2TN51YD919 (Hypoxanthine)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Corpus Striatum/*drug effects/enzymology/physiopathology
MH  - Enzyme Inhibitors/pharmacology
MH  - Free Radicals/metabolism
MH  - Hypoxanthine/*pharmacology
MH  - Lesch-Nyhan Syndrome/metabolism
MH  - Membrane Proteins/chemistry/drug effects/metabolism
MH  - Models, Neurological
MH  - Neurons/chemistry/*drug effects/enzymology
MH  - Oxidative Stress/*drug effects/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium-Potassium-Exchanging ATPase/*drug effects/metabolism
MH  - Subcellular Fractions
MH  - Sulfhydryl Compounds/metabolism
MH  - Synaptic Membranes/drug effects/enzymology
MH  - Synaptic Transmission/drug effects/physiology
MH  - Thiobarbituric Acid Reactive Substances/metabolism
EDAT- 2005/04/15 09:00
MHDA- 2005/07/01 09:00
CRDT- 2005/04/15 09:00
PHST- 2004/05/13 00:00 [received]
PHST- 2005/02/01 00:00 [revised]
PHST- 2005/02/04 00:00 [accepted]
PHST- 2005/04/15 09:00 [pubmed]
PHST- 2005/07/01 09:00 [medline]
PHST- 2005/04/15 09:00 [entrez]
AID - S0006-8993(05)00245-3 [pii]
AID - 10.1016/j.brainres.2005.02.012 [doi]
PST - ppublish
SO  - Brain Res. 2005 Apr 18;1041(2):198-204. doi: 10.1016/j.brainres.2005.02.012.