PMID- 15830096
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20050414
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 57
IP  - 2
DP  - 2005 Apr 25
TI  - Induction of rat neural stem cells into oligodendrocyte precursor cells.
PG  - 132-8
AB  - We have previously established a culture method to isolate and cultivate neural 
      stem cells (NSCs) derived from the rat embryonic brain and spinal cord. In the 
      present study, we demonstrate that the spinal cord-derived NSCs can be induced to 
      differentiate into oligodendrocyte precursor cells (OPCs) with a combined 
      treatment composed of (1) conditioned medium collected from B104 neuroblastoma 
      cells (B104CM) and (2) basic fibroblast growth factor (bFGF, 10 ng/ml). After 
      induction, over 95% of the cells displayed bipolar or tri-polar morphology and 
      expressed A2B5 and platelet derived growth factor receptor-alpha (PDGFR-alpha), 
      markers that are specific for OPCs. Among PDGFR-alpha positive OPCs, only a few 
      cells expressed glia fibrillary acidic protein (GFAP) and none expressed 
      beta-tubulin III. In the presence of B104CM and bFGF, OPCs proliferated rapidly, 
      formed spheres, expanded for multiple passages, and maintained their phenotypic 
      properties. Upon withdrawal of B104CM and bFGF, these cells differentiated into 
      either O4/GlaC-positive oligodendrocytes (OLs) or GFAP- and A2B5-positive type-2 
      astrocytes. Our results indicate that NSCs can be induced to differentiate into 
      OPCs that possess properties of self-renewal and differentiation into 
      oligodendrocytes and type-2 astrocytes, a property similar to that of O-2A 
      progenitor cells. The OPCs can be maintained in an undifferentiated state over 
      multiple divisions as long as both B104CM and bFGF are present in the medium. 
      Thus, large quantity of OPCs can be obtained through this method for potential 
      therapeutical interventions for various neurological degenerative diseases.
FAU - Fu, Sai-Li
AU  - Fu SL
AD  - Department of Neurobiology, Shanghai Second Medical University, Shanghai 200025, 
      China.
FAU - Hu, Jian-Guo
AU  - Hu JG
FAU - Li, Ying
AU  - Li Y
FAU - Yin, Lan
AU  - Yin L
FAU - Jin, Jian-Qiang
AU  - Jin JQ
FAU - Xu, Xiao-Ming
AU  - Xu XM
FAU - Lu, Pei-Hua
AU  - Lu PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Hexanones)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 111050-72-7 (1-((3,5-dichloro)-2,6-dihydroxy-4-methoxyphenyl)-1-hexanone)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Embryo, Mammalian
MH  - Female
MH  - Fibroblast Growth Factor 2/physiology
MH  - Hexanones
MH  - Neural Stem Cells/*cytology
MH  - Neuroblastoma/*pathology
MH  - Oligodendroglia/*cytology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
EDAT- 2005/04/15 09:00
MHDA- 2012/06/29 06:00
CRDT- 2005/04/15 09:00
PHST- 2005/04/15 09:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
PHST- 2005/04/15 09:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2005 Apr 25;57(2):132-8.