PMID- 15831287
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20091119
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 83 Suppl 1
DP  - 2005 Apr
TI  - Increases in phosphorylation of SAPK/JNK and p38MAPK correlate negatively with 
      mouse embryo development after culture in different media.
PG  - 1144-54
AB  - OBJECTIVE: To test whether signal transduction proteins that mediate stress may 
      be used to detect responses of embryos to different media in a prospective 
      randomized study. DESIGN: Controlled laboratory study. SETTING: None. PATIENT(S): 
      None. INTERVENTION(S): Mouse embryos isolated at E3.5 (3.5 days after 
      fertilization) or E1.5 were cultured in different media for 24 hours or 72 hours, 
      respectively. Expression of p38 mitogen activated protein kinases (MAPKs) and 
      stress-activated protein kinase/Jun kinase (SAPK/JNK) phosphoproteins in the 
      mouse embryo and their correlation with preimplantation development were studied. 
      MAIN OUTCOME MEASURE(S): [1] In E3.5 embryos, SAPK/JNK and p38MAPK are 
      phosphorylated at different levels in different media after 24 hours, with Ham's 
      F10+BSA and M-16 having the highest intensity of both SAPK/JNK and p38MAPK 
      phosphorylation and Quinn's cleavage medium and potassium simplex optimized 
      medium supplemented with amino acids (KSOM+AA) the lowest intensity. [2] The 
      stress-induced increase in phosphorylation of SAPK/JNK and p38MAPK appears to be 
      post-translational in embryos. [3] The intensity of SAPK/JNK phosphorylation 
      measured at E1.5+72 hours culture is inversely correlated with 4-cell/compaction 
      rate, morula formation rate, blastocyst formation rate, and hatching rate. 
      RESULT(S): SAPK/JNK and p38MAPK phosphoprotein levels, but not all forms of 
      protein, are affected during culture of preimplantation embryos in seven 
      different media. During culture, the rate of progress to four developmental 
      events was assayed and each rate was inversely proportional to the level of 
      SAPK/JNK phosphorylation measured by immunocytochemical means or Western blot 
      analysis at the end of culture. CONCLUSION(S): Culture stresses embryos; 
      different media exert different levels of stress on the embryos. There is a 
      negative correlation between the amount of stress and the development rate. Taken 
      together, the data suggest that SAPK/JNK phosphorylation may constitute a measure 
      of homeostatic response to negative stimuli of media.
FAU - Wang, Yingchun
AU  - Wang Y
AD  - C.S. Mott Center for Human Growth and Development, Department of Obstetrics and 
      Gynecology, Hutzel Hospital, Wayne State University School of Medicine, 275 East 
      Hancock, Detroit, MI 48201, USA.
FAU - Puscheck, Elizabeth E
AU  - Puscheck EE
FAU - Lewis, Jennifer J
AU  - Lewis JJ
FAU - Trostinskaia, Anna B
AU  - Trostinskaia AB
FAU - Wang, Fangfei
AU  - Wang F
FAU - Rappolee, Daniel A
AU  - Rappolee DA
LA  - eng
GR  - R01 HD 40972 A/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Buffers)
RN  - 0 (Culture Media)
RN  - 0 (Isotonic Solutions)
RN  - 0 (nutrient mixture F-10 Ham)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Buffers
MH  - Culture Media/*pharmacology
MH  - *Embryo Culture Techniques
MH  - Embryonic Development/drug effects/*physiology
MH  - Female
MH  - Isotonic Solutions/pharmacology
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - MAP Kinase Signaling System/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phosphorylation
MH  - Pregnancy
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
OTO - NASA
OT  - NASA Discipline Developmental Biology
OT  - Non-NASA Center
FIR - Rappolee, D A
IR  - Rappolee DA
IRAD- Wayne St U, Detroit, MI
GN  - NASA: Grant numbers: NASA NAG 2-150309.
EDAT- 2005/04/16 09:00
MHDA- 2005/06/09 09:00
CRDT- 2005/04/16 09:00
PHST- 2004/04/05 00:00 [received]
PHST- 2004/08/18 00:00 [revised]
PHST- 2004/08/18 00:00 [accepted]
PHST- 2005/04/16 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/04/16 09:00 [entrez]
AID - S0015-0282(04)03213-3 [pii]
AID - 10.1016/j.fertnstert.2004.08.038 [doi]
PST - ppublish
SO  - Fertil Steril. 2005 Apr;83 Suppl 1:1144-54. doi: 
      10.1016/j.fertnstert.2004.08.038.