PMID- 15831956
OWN - NLM
STAT- MEDLINE
DCOM- 20050531
LR  - 20200306
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 86
IP  - Pt 5
DP  - 2005 May
TI  - Analysis of porcine reproductive and respiratory syndrome virus attachment and 
      internalization: distinctive roles for heparan sulphate and sialoadhesin.
PG  - 1441-1445
LID - 10.1099/vir.0.80675-0 [doi]
AB  - Heparan sulphate and sialoadhesin were previously identified on porcine 
      macrophages as receptors for porcine reproductive and respiratory syndrome virus 
      (PRRSV). In this study, the exact role and cooperation of heparan sulphate and 
      sialoadhesin during PRRSV attachment and internalization was analysed. It was 
      observed that both heparan sulphate and sialoadhesin mediate PRRSV attachment and 
      that only these two receptors are involved in attachment. Analysis of attachment 
      kinetics of PRRSV to macrophages revealed that early attachment is mediated 
      mainly via an interaction with heparan sulphate, followed by a gradual increase 
      in interaction with sialoadhesin. By using wild-type CHO and CHO deficient in 
      heparan sulphate expression, it was shown that heparan sulphate alone is 
      sufficient to mediate PRRSV attachment, but not entry, and that heparan sulphate 
      is not necessary for sialoadhesin to function as a PRRSV internalization 
      receptor, but enhances the interaction of the virus with sialoadhesin.
FAU - Delputte, P L
AU  - Delputte PL
AD  - Laboratory of Virology, Department of Virology, Parasitology and Immunology, 
      Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 
      Merelbeke, Belgium.
FAU - Costers, S
AU  - Costers S
AD  - Laboratory of Virology, Department of Virology, Parasitology and Immunology, 
      Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 
      Merelbeke, Belgium.
FAU - Nauwynck, H J
AU  - Nauwynck HJ
AD  - Laboratory of Virology, Department of Virology, Parasitology and Immunology, 
      Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 
      Merelbeke, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Virus)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
RN  - 9050-30-0 (Heparitin Sulfate)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Heparitin Sulfate/*physiology
MH  - Kinetics
MH  - Macrophages/*virology
MH  - Membrane Glycoproteins/*physiology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Receptors, Immunologic/*physiology
MH  - Receptors, Virus/*physiology
MH  - Sialic Acid Binding Ig-like Lectin 1
MH  - Swine
EDAT- 2005/04/16 09:00
MHDA- 2005/06/01 09:00
CRDT- 2005/04/16 09:00
PHST- 2005/04/16 09:00 [pubmed]
PHST- 2005/06/01 09:00 [medline]
PHST- 2005/04/16 09:00 [entrez]
AID - 10.1099/vir.0.80675-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2005 May;86(Pt 5):1441-1445. doi: 10.1099/vir.0.80675-0.