PMID- 15832422
OWN - NLM
STAT- MEDLINE
DCOM- 20050609
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 16
DP  - 2005 Apr 28
TI  - Expression of cellular FLICE-inhibitory protein and its association with p53 
      mutation in colon cancer.
PG  - 2482-5
AB  - AIM: To investigate the expression of cellular FLICE (Fas associated death 
      domain-like IL-1beta-converting enzyme)-inhibitory protein (c-FLIP) and its 
      association with p53 mutation in colon cancer. METHODS: Immunohistochemical 
      staining of c-FLIP and mutant p53 by using specific antibodies was performed by 
      the standard streptavidin-peroxidase technique for 45 colon cancer tissue samples 
      with matched normal tissues. Semi-quantitative reverse transcriptional (RT)-PCR 
      was used to measure c-FLIP mRNA levels. t-test statistical method was used in 
      data analyses. RESULTS: c-FLIP mRNA was expressed in all colon cancer tissues and 
      its level (0.63+/-0.12) was significantly higher than that in normal tissues 
      (0.38+/-0.10, P<0.01). Immuno-histochemically, c-FLIP protein was also expressed 
      in all colon cancers (45/45) and 71.1% (32/45) showed an intense immunostaining, 
      in contrast, 93.3% (42/45) of normal colonic mucosa showed positive staining and 
      none of them immunostained intensely. The quantity of c-FLIP protein was 
      significantly higher in cancer tissues than in normal mucosa (7.04+/-1.20 vs 
      5.21+/-0.86, P<0.01). Positive staining of mutant p53 protein was found in 60% 
      (27/45) colon cancers. c-FLIP mRNA level was decreased in p53 positive group 
      compared with p53 negative cancer tissues (0.59+/-0.13 vs 0.69+/-0.14, P<0.01), 
      but c-FLIP protein had a significantly higher level in p53 positive cancer 
      tissues than in negative ones (7.57+/-1.30 vs 6.25+/-1.27, P<0.01). CONCLUSION: 
      c-FLIP is specially overexpressed in colon cancers and it might contribute to 
      carcinogenesis of normal colonic mucosa. p53 may exert transcriptional 
      upregulation effects on c-FLIP gene and more potent effects on promoting the 
      degradation of c-FLIP protein.
FAU - Zhou, Xiao-Dong
AU  - Zhou XD
AD  - Department of Gastroenterology, Renmin Hospital of Wuhan University, Jiefang road 
      238, Wuhan 430060, Hubei Province, China. zhouxd7612@hotmail.com
FAU - Yu, Jie-Ping
AU  - Yu JP
FAU - Chen, Hong-Xia
AU  - Chen HX
FAU - Yu, Hong-Gang
AU  - Yu HG
FAU - Luo, He-Sheng
AU  - Luo HS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (CASP8 and FADD-Like Apoptosis Regulating Protein)
RN  - 0 (CFLAR protein, human)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - CASP8 and FADD-Like Apoptosis Regulating Protein
MH  - Colonic Neoplasms/*genetics/metabolism/*physiopathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunohistochemistry
MH  - Intracellular Signaling Peptides and Proteins/*genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - RNA, Messenger/analysis
MH  - Tumor Suppressor Protein p53/*genetics/metabolism
PMC - PMC4305639
EDAT- 2005/04/16 09:00
MHDA- 2005/06/10 09:00
PMCR- 2005/04/28
CRDT- 2005/04/16 09:00
PHST- 2005/04/16 09:00 [pubmed]
PHST- 2005/06/10 09:00 [medline]
PHST- 2005/04/16 09:00 [entrez]
PHST- 2005/04/28 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i16.2482 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 Apr 28;11(16):2482-5. doi: 10.3748/wjg.v11.i16.2482.