PMID- 15832427
OWN - NLM
STAT- MEDLINE
DCOM- 20050609
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 16
DP  - 2005 Apr 28
TI  - Profiling of differentially expressed chemotactic-related genes in MCP-1 treated 
      macrophage cell line using human cDNA arrays.
PG  - 2508-12
AB  - AIM: To study the global gene expression of chemotactic genes in macrophage line 
      U937 treated with human monocyte chemoattractant protein-1 (MCP-1) through the 
      use of ExpreeChipHO2 cDNA array. METHODS: Total RNA was extracted from MCP-1 
      treated macrophage line U937 and normal U937 cells, reversely transcribed to 
      cDNA, and then screened in parallel with HO2 human cDNA array chip. The scanned 
      result was additionally validated using RT-PCR. RESULTS: The result of cDNA array 
      showed that one chemotactic-related gene was up-regulated more than two-fold 
      (RANTES) and seven chemotactic-related genes were down-regulated more than 
      two-fold (CCR1, CCR5, ccl16, GRObeta, GROgamma, IL-8 and granulocyte chemotactic 
      protein 2) in MCP-1 treated U937 cells at mRNA level. RT-PCR analysis of four of 
      these differentially expressed genes gave results consistent with cDNA array 
      findings. CONCLUSION: MCP-1 could influence some chemokine and receptor 
      expressions in macrophages in vitro. MCP-1 mainly down-regulates the expression 
      of chemotactic genes influencing neutrophilic granulocyte expression (GRObeta, 
      GROgamma, IL-8 and granulocyte chemotactic protein 2), and the mRNA level of 
      CCR5, which plays a critical role in many disorders and illnesses.
FAU - Bian, Guang-Xing
AU  - Bian GX
AD  - Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical 
      University, Shanghai 200433, China.
FAU - Miao, Hong
AU  - Miao H
FAU - Qiu, Lei
AU  - Qiu L
FAU - Cao, Dong-Mei
AU  - Cao DM
FAU - Guo, Bao-Yu
AU  - Guo BY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Chemokine CCL2/*pharmacology
MH  - Chemotaxis/drug effects/*genetics/immunology
MH  - Gene Expression/immunology
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Macrophages/cytology/drug effects/*physiology
MH  - Neutrophils/physiology
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - U937 Cells
PMC - PMC4305644
EDAT- 2005/04/16 09:00
MHDA- 2005/06/10 09:00
PMCR- 2005/04/28
CRDT- 2005/04/16 09:00
PHST- 2005/04/16 09:00 [pubmed]
PHST- 2005/06/10 09:00 [medline]
PHST- 2005/04/16 09:00 [entrez]
PHST- 2005/04/28 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i16.2508 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 Apr 28;11(16):2508-12. doi: 10.3748/wjg.v11.i16.2508.