PMID- 15833104
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20061115
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 53
IP  - 5
DP  - 2005 May
TI  - Effect of bacterial virulence on IL-18 expression in the amnion infected with 
      Escherichia coli.
PG  - 255-60
AB  - PROBLEM: The upregulation of inflammatory substances threatens pregnancy. 
      Interleukin-18 (IL-18) is elevated in women who miscarried. The purpose of this 
      study was to develop a pig model of chorioamnionitis to study the effect of 
      bacterial virulence on IL-18 response in experimentally infected amnion. METHOD 
      OF STUDY: A total of 20,000 colony-forming units of Escherichia coli (an 
      enteropathogenic O55 strain, EPEC or O86 non-pathogenic strain) were administered 
      into the amniotic cavity of pig fetuses at 70% of gestation for 10 hr. Fetal 
      amniotic fluid samples were analyzed for IL-18 levels by enzyme-linked 
      immunosorbent assay. The expression of IL-18 was studied also by 
      immunohistochemistry on cryostat sections through amniotic membranes and 
      pathological changes were observed by electron microscopy. RESULTS: Both E. coli 
      strains propagated in amniotic fluids and reached similar counts. Only EPEC, 
      however, caused a significant increase of IL-18 amniotic fluid levels (P < 0.001) 
      and cytokine expression in the amniotic epithelium. CONCLUSIONS: The levels of 
      IL-18 in infected amniotic fluids correlated with bacterial virulence and 
      pathological changes in the amnion.
FAU - Splichalova, Alla
AU  - Splichalova A
AD  - Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of 
      Sciences of the Czech Republic, 549 22 Novy Hradek, Czech Republic. 
      splichal@biomed.cas.cz
FAU - Trebichavsky, Ilja
AU  - Trebichavsky I
FAU - Muneta, Yoshihiro
AU  - Muneta Y
FAU - Mori, Yasuyuki
AU  - Mori Y
FAU - Splichal, Igor
AU  - Splichal I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Amnion/*microbiology/ultrastructure
MH  - Animals
MH  - Escherichia coli/*pathogenicity
MH  - Escherichia coli Infections/*metabolism
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Immunohistochemistry
MH  - Interleukin-18/biosynthesis/*genetics
MH  - Microscopy, Electron, Transmission
MH  - Pregnancy
MH  - Swine
MH  - Virulence
EDAT- 2005/04/19 09:00
MHDA- 2005/08/03 09:00
CRDT- 2005/04/19 09:00
PHST- 2005/04/19 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2005/04/19 09:00 [entrez]
AID - AJI273 [pii]
AID - 10.1111/j.1600-0897.2005.00273.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2005 May;53(5):255-60. doi: 
      10.1111/j.1600-0897.2005.00273.x.