PMID- 15833370
OWN - NLM
STAT- MEDLINE
DCOM- 20050705
LR  - 20220129
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 162
IP  - 1-2
DP  - 2005 May
TI  - Inhibition of acetylcholine receptor function by seronegative myasthenia gravis 
      non-IgG factor correlates with desensitisation.
PG  - 149-56
AB  - 15% of myasthenia gravis (MG) patients do not have antibodies against the 
      acetylcholine receptor (AChR). Some of these "seronegative" MG patients have 
      antibodies against muscle specific kinase (MuSK), and many have a non-IgG factor 
      that acutely inhibits AChR function in a muscle-like cell line, CN21. Here we 
      show, using mainly one plasma negative for both AChR and MuSK antibodies, that 
      the inhibitory effect of the non-IgG fraction correlates well with the 
      desensitisation caused by 100 microM nicotine, and is found also when AChRs are 
      expressed in a non-muscle cell line (HEK). Moreover, a similar effect was seen 
      with M3C7-a monoclonal antibody against human AChR. The results suggest that, 
      rather than acting indirectly as previously proposed, the SNMG factor may bind 
      directly to an allosteric site that induces or enhances AChR desensitisation.
FAU - Spreadbury, Ian
AU  - Spreadbury I
AD  - Neurosciences Group, Weatherall Institute of Molecular Medicine, John Radcliffe 
      Hospital, Headington, Oxford OX3 9DS, United Kingdom.
FAU - Kishore, Uday
AU  - Kishore U
FAU - Beeson, David
AU  - Beeson D
FAU - Vincent, Angela
AU  - Vincent A
LA  - eng
GR  - G117/490/MRC_/Medical Research Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Antibodies)
RN  - 0 (Autoantibodies)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Receptors, Cholinergic)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adult
MH  - Antibodies/pharmacology
MH  - Autoantibodies/blood/immunology/*pharmacology
MH  - Cell Line, Tumor
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Potentials/drug effects/physiology
MH  - Myasthenia Gravis/*immunology
MH  - Nicotine/pharmacology
MH  - Nicotinic Agonists/pharmacology
MH  - Patch-Clamp Techniques/methods
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Receptors, Cholinergic/*drug effects/immunology/metabolism/physiology
MH  - Rhabdomyosarcoma
EDAT- 2005/04/19 09:00
MHDA- 2005/07/06 09:00
CRDT- 2005/04/19 09:00
PHST- 2004/08/20 00:00 [received]
PHST- 2004/10/26 00:00 [revised]
PHST- 2005/01/06 00:00 [accepted]
PHST- 2005/04/19 09:00 [pubmed]
PHST- 2005/07/06 09:00 [medline]
PHST- 2005/04/19 09:00 [entrez]
AID - S0165-5728(05)00014-7 [pii]
AID - 10.1016/j.jneuroim.2005.01.009 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2005 May;162(1-2):149-56. doi: 10.1016/j.jneuroim.2005.01.009.