PMID- 15833768
OWN - NLM
STAT- MEDLINE
DCOM- 20060223
LR  - 20240312
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Print)
IS  - 0892-6638 (Linking)
VI  - 19
IP  - 9
DP  - 2005 Jul
TI  - Thrombospondin-1 up-regulates expression of cell adhesion molecules and promotes 
      monocyte binding to endothelium.
PG  - 1158-60
AB  - Expression of cell adhesion molecules (CAM) responsible for leukocyte-endothelium 
      interactions plays a crucial role in inflammation and atherogenesis. 
      Up-regulation of vascular CAM-1 (VCAM-1), intracellular CAM-1 (ICAM-1), and 
      E-selectin expression promotes monocyte recruitment to sites of injury and is 
      considered to be a critical step in atherosclerotic plaque development. Factors 
      that trigger this initial response are not well understood. As platelet 
      activation not only promotes thrombosis but also early stages of atherogenesis, 
      we considered the role of thrombospondin-1 (TSP-1), a matricellular protein 
      released in abundance from activated platelets and accumulated in sites of 
      vascular injury, as a regulator of CAM expression. TSP-1 induced expression of 
      VCAM-1 and ICAM-1 on endothelium of various origins, which in turn, resulted in a 
      significant increase of monocyte attachment. This effect could be mimicked by a 
      peptide derived from the C-terminal domain of TSP-1 and known to interact with 
      CD47 on the cell surface. The essential role of CD47 in the cellular responses to 
      TSP-1 was demonstrated further using inhibitory antibodies and knockdown of CD47 
      with small interfering RNA. Furthermore, we demonstrated that secretion of 
      endogenous TSP-1 and its interaction with CD47 on the cell surface mediates 
      endothelial response to the major proinflammatory agent, tumor necrosis factor 
      alpha (TNF-alpha). Taken together, this study identifies a novel mechanism 
      regulating CAM expression and subsequent monocyte binding to endothelium, which 
      might influence the development of anti-atherosclerosis therapeutic strategies.
FAU - Narizhneva, Natalya V
AU  - Narizhneva NV
AD  - Department of Molecular Cardiology, Cleveland Clinic Foundation, Cleveland, Ohio 
      44195, USA.
FAU - Razorenova, Olga V
AU  - Razorenova OV
FAU - Podrez, Eugene A
AU  - Podrez EA
FAU - Chen, Juhua
AU  - Chen J
FAU - Chandrasekharan, Unni M
AU  - Chandrasekharan UM
FAU - DiCorleto, Paul E
AU  - DiCorleto PE
FAU - Plow, Edward F
AU  - Plow EF
FAU - Topol, Eric J
AU  - Topol EJ
FAU - Byzova, Tatiana V
AU  - Byzova TV
LA  - eng
GR  - R01 HL071625/HL/NHLBI NIH HHS/United States
GR  - RR-00080/RR/NCRR NIH HHS/United States
GR  - HL071625./HL/NHLBI NIH HHS/United States
GR  - M01 RR000080/RR/NCRR NIH HHS/United States
GR  - P50 HL077107/HL/NHLBI NIH HHS/United States
GR  - P50 HL77107/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050415
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (CD47 Antigen)
RN  - 0 (CD47 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (E-Selectin)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Thrombospondin 1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Atherosclerosis/etiology
MH  - CD47 Antigen/physiology
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/*genetics
MH  - Cells, Cultured
MH  - E-Selectin/genetics
MH  - Endothelial Cells/cytology/*metabolism
MH  - *Gene Expression Regulation/drug effects
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Monocytes/*physiology
MH  - NF-kappa B/metabolism
MH  - RNA, Small Interfering/pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Thrombospondin 1/*physiology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Up-Regulation
MH  - Vascular Cell Adhesion Molecule-1/genetics
PMC - PMC1569946
MID - NIHMS9154
EDAT- 2005/04/19 09:00
MHDA- 2006/02/24 09:00
PMCR- 2008/01/28
CRDT- 2005/04/19 09:00
PHST- 2005/04/19 09:00 [pubmed]
PHST- 2006/02/24 09:00 [medline]
PHST- 2005/04/19 09:00 [entrez]
PHST- 2008/01/28 00:00 [pmc-release]
AID - 04-3310fje [pii]
AID - 10.1096/fj.04-3310fje [doi]
PST - ppublish
SO  - FASEB J. 2005 Jul;19(9):1158-60. doi: 10.1096/fj.04-3310fje. Epub 2005 Apr 15.