PMID- 15836753
OWN - NLM
STAT- MEDLINE
DCOM- 20050711
LR  - 20131121
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 35
IP  - 4
DP  - 2005 Apr
TI  - Increased responsiveness of basophils of patients with chronic urticaria to sera 
      but hypo-responsiveness to other stimuli.
PG  - 456-60
AB  - BACKGROUND: Although it has been shown that basophils from patients with chronic 
      ordinary urticaria (CU) are less responsive than normal basophils when stimulated 
      with anti-IgE, very few studies have examined the response of those cells to 
      alternative stimuli. OBJECTIVE: To compare releasability between basophils from 
      healthy donors and patients with CU. METHODS: We examined the response of 
      IL-3-treated basophils from healthy donors, atopic controls and CU patients to 
      anti-IgE, monocyte chemoattractant protein-1 (MCP-1), bradykinin, C5a and to sera 
      obtained from other urticaria patients and normal controls. We also compared the 
      response of basophils from CU patients whose sera activate normal basophils 
      (autoimmune CU) from those who do not (idiopathic CU). RESULTS: Basophils of CU 
      patients release significantly less histamine than basophils of normal controls 
      when stimulated with anti-IgE, and to a lesser degree with C5a. No differences 
      were observed when basophils from patients were incubated with Bradykinin or 
      MCP-1. However, when basophils of CU patients were incubated with sera from other 
      CU patients or even normal sera, we found significantly higher histamine release 
      compared with the response of basophils from normal donors. We could not 
      distinguish responsiveness of basophils of patients with chronic autoimmune 
      urticaria from patients with chronic idiopathic urticaria. CONCLUSION: Basophils 
      of patients with chronic idiopathic urticaria and chronic autoimmune urticaria 
      are hypo-responsive to anti-IgE and C5a, normally responsive to MCP-1 or 
      bradykinin, and hyper-responsive to serum. The serum factor to which a response 
      has not yet been identified; however, basophils of patients with chronic 
      urticaria, in general, appear to have abnormal regulation of signaling pathways.
FAU - Luquin, E
AU  - Luquin E
AD  - Department of Allergy and Clinical Immunology, Clinica Universitaria, Universidad 
      de Navarra, Pamplona, Spain.
FAU - Kaplan, A P
AU  - Kaplan AP
FAU - Ferrer, M
AU  - Ferrer M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Autoantibodies)
RN  - 0 (Chemokine CCL2)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 80295-54-1 (Complement C5a)
RN  - S8TIM42R2W (Bradykinin)
SB  - IM
MH  - Antibodies, Anti-Idiotypic/immunology
MH  - Autoantibodies/immunology
MH  - Basophils/*immunology
MH  - Bradykinin/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/immunology
MH  - Chronic Disease
MH  - Complement C5a/immunology
MH  - Histamine Release/immunology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Urticaria/*immunology
EDAT- 2005/04/20 09:00
MHDA- 2005/07/12 09:00
CRDT- 2005/04/20 09:00
PHST- 2005/04/20 09:00 [pubmed]
PHST- 2005/07/12 09:00 [medline]
PHST- 2005/04/20 09:00 [entrez]
AID - CEA2212 [pii]
AID - 10.1111/j.1365-2222.2005.02212.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2005 Apr;35(4):456-60. doi: 10.1111/j.1365-2222.2005.02212.x.