PMID- 15836845
OWN - NLM
STAT- MEDLINE
DCOM- 20050602
LR  - 20151119
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 125
IP  - 1
DP  - 2005 May 1
TI  - L-carnitine could not improve hepatic warm ischemia-reperfusion injury despite 
      ameliorated blood flow.
PG  - 16-22
AB  - BACKGROUND: Carnitine is applied to ameliorate ischemia-reperfusion (I/R) injury 
      of several organs. However, application to hepatic I/R injury is not frequently 
      reported. The aim of this study was to elucidate the effect of exogenous 
      carnitine administration to ameliorate the warm hepatic I/R injury. MATERIALS AND 
      METHODS: Male Wistar rats were divided into two groups, a carnitine group 
      (Car);100 mg/kg of l-carnitine administration and a control group (C); vehicle 
      administration. Thirty minutes after administration, the left hepatic lobes were 
      given 60-min ischemia and then reperfused. Plasma alanine aminotransferase (ALT), 
      aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), tumor necrosis 
      factor (TNF)-alpha, and lipoperoxides (LPO) were measured. Hepatic adenosine 
      triphosphate (ATP) concentration was also measured. The hepatic blood flow was 
      estimated using a Laser Doppler. The presence of apoptosis in the livers was 
      evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) staining. RESULTS: In group Car, the blood flow of the left 
      hepatic lobes was better recovered during the reperfusion period than in group C 
      (P < 0.0001). Plasma levels of ALT, AST, GLDH, and TNF-alpha at 1 h after 
      reperfusion were not significantly different between the groups. Although there 
      were no statistical significances, ALT, AST, and TNF-alpha levels in group Car at 
      24 h after reperfusion tended to be higher than in group C. Plasma LPO levels 
      were not different between the two groups. Also hepatic ATP concentration was not 
      different between the two groups. TUNEL positive liver cells were visible only in 
      group Car at 24 h after reperfusion, but not in the controls. CONCLUSIONS: 
      Although carnitine administration improved the hepatic blood flow during the 
      reperfusion period, we could not demonstrate a protective effect to the hepatic 
      warm I/R injury.
FAU - Yonezawa, Kei
AU  - Yonezawa K
AD  - Department of Surgery, Division of Surgical Research, University of Bonn, Bonn, 
      Germany.
FAU - Tolba, Rene H
AU  - Tolba RH
FAU - Wetter, Axel
AU  - Wetter A
FAU - Yamamoto, Yuzo
AU  - Yamamoto Y
FAU - Yamaoka, Yoshio
AU  - Yamaoka Y
FAU - Minor, Thomas
AU  - Minor T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Blood Glucose)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - S7UI8SM58A (Carnitine)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Apoptosis
MH  - Blood Glucose/analysis
MH  - Carnitine/*therapeutic use
MH  - L-Lactate Dehydrogenase/blood
MH  - Liver/*blood supply
MH  - Liver Circulation/*drug effects
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*drug therapy
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2005/04/20 09:00
MHDA- 2005/06/03 09:00
CRDT- 2005/04/20 09:00
PHST- 2004/06/08 00:00 [received]
PHST- 2004/10/25 00:00 [revised]
PHST- 2004/11/21 00:00 [accepted]
PHST- 2005/04/20 09:00 [pubmed]
PHST- 2005/06/03 09:00 [medline]
PHST- 2005/04/20 09:00 [entrez]
AID - S0022-4804(04)00685-7 [pii]
AID - 10.1016/j.jss.2004.11.016 [doi]
PST - ppublish
SO  - J Surg Res. 2005 May 1;125(1):16-22. doi: 10.1016/j.jss.2004.11.016.