PMID- 15838903
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20131121
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 5
IP  - 6
DP  - 2005 Apr
TI  - Proteomic analysis of the proteins expressed by hydrogen peroxide treated 
      cultured human dermal microvascular endothelial cells.
PG  - 1507-19
AB  - Reactive oxygen species (ROS) have been traditionally regarded as toxic 
      by-products of aerobic metabolism. However, ROS also act as intracellular 
      signaling molecules and can mediate phenotypes in vascular endothelial cells, 
      which may be physiological or pathological in nature. To clarify the molecular 
      mechanisms of ROS signaling, we examined hydrogen peroxide (H(2)O(2))-responsive 
      proteins in cultured human dermal microvascular endothelial cells (HMVEC) using 
      proteomic tools. Protein expression in HMVEC was studied after they had been 
      exposed to low- and high-levels of H(2)O(2) for various times, and intracellular 
      ROS production was examined by flow cytometer and UV spectrophotometer. Proteins 
      obtained from dose- and time-dependent series were separated by two-dimensional 
      gel electrophoresis and tentatively identified by matrix-assisted laser 
      desorption-time of flight mass spectrometry, by matching the tryptic mass maps 
      obtained with entries in the NCBI and Swiss-Prot protein sequence database. At 
      least 163 proteins were changed by H(2)O(2), and 60 proteins were identified. 
      Oxidative stress triggered dramatic change in the expression of proteins in 
      primary microvessel endothelial cells, and their mapping to cellular process 
      provided a view of the ubiquitous cellular changes elicited by H(2)O(2). These 
      results could provide a framework for the understanding of the mechanisms of 
      cellular redox homeostasis and H(2)O(2) metabolism in microendothelium 
      environment in various biological processes as well as pathological conditions.
FAU - Ha, Moon Kyung
AU  - Ha MK
AD  - Department of Dermatology and Cutaneous Biology Research Institute, Seoul, Korea.
FAU - Chung, Kee Yang
AU  - Chung KY
FAU - Bang, Dongsik
AU  - Bang D
FAU - Park, Yoon Kee
AU  - Park YK
FAU - Lee, Kwang Hoon
AU  - Lee KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Proteome)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytoplasm/metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Endothelial Cells/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Hydrogen Peroxide/*pharmacology
MH  - Microcirculation/cytology/metabolism
MH  - Mitochondria/drug effects/metabolism
MH  - Molecular Sequence Data
MH  - Proteome/*biosynthesis
MH  - Reactive Oxygen Species/metabolism
MH  - Skin/*blood supply
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spectrophotometry, Ultraviolet
MH  - beta-Galactosidase/metabolism
EDAT- 2005/04/20 09:00
MHDA- 2005/08/03 09:00
CRDT- 2005/04/20 09:00
PHST- 2005/04/20 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2005/04/20 09:00 [entrez]
AID - 10.1002/pmic.200401043 [doi]
PST - ppublish
SO  - Proteomics. 2005 Apr;5(6):1507-19. doi: 10.1002/pmic.200401043.