PMID- 15840426
OWN - NLM
STAT- MEDLINE
DCOM- 20050601
LR  - 20141120
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 210
IP  - 2-3
DP  - 2005 Jun 1
TI  - Urinary metabolites of di-n-octyl phthalate in rats.
PG  - 123-33
AB  - Di-n-octyl phthalate (DnOP) is a plasticizer used in polyvinyl chloride plastics, 
      cellulose esters, and polystyrene resins. The metabolism of DnOP results in the 
      hydrolysis of one ester linkage to produce mono-n-octyl phthalate (MnOP), which 
      subsequently metabolizes to form oxidative metabolites. We investigated the 
      toxicokinetics of DnOP in adult female Sprague-Dawley rats by monitoring the 
      excretion of DnOP metabolites in urine after oral administration of DnOP (300 
      mg/kg). By using authentic standards, the presence of urinary phthalic acid (PA), 
      MnOP, and the major DnOP metabolite, mono-(3-carboxypropyl) phthalate (MCPP) was 
      clearly established. Furthermore, we identified five additional urinary DnOP 
      oxidative metabolites based on their chromatographic behavior and mass 
      spectrometric fragmentation pattern. These DnOP oxidative metabolites, are 
      postulated to be mono-carboxymethyl phthalate (MCMP), mono-(5-carboxy-n-pentyl) 
      phthalate (MCPeP), mono-(7-carboxy-n-heptyl) phthalate (MCHpP), and isomers of 
      mono-hydroxy-n-octyl phthalate (MHOP) (e.g., mono-(7-hydroxy-n-octyl) phthalate) 
      and of mono-oxo-n-octyl phthalate (MOOP) (e.g., mono-(7-oxo-n-octyl) phthalate). 
      The urinary excretion of DnOP metabolites followed a biphasic excretion pattern. 
      The metabolite levels decreased significantly after the first day of DnOP 
      administration although MCPP, MCHpP, MHOP, and MOOP were detectable after 4 days. 
      We also studied the in vitro metabolism of DnOP and MnOP by rat liver microsomes. 
      DnOP produced MnOP, MHOP, and PA in vitro whereas, MnOP produced MHOP and PA in 
      vitro at detectable levels.
FAU - Silva, Manori J
AU  - Silva MJ
AD  - Division of Laboratory Sciences, National Center for Environmental Health, 
      Centers for Disease Control and Prevention, Mailstop F17, 4770 Buford Hwy NE, 
      Atlanta, GA 30341, USA. zca2@cdc.gov
FAU - Kato, Kayoko
AU  - Kato K
FAU - Gray, Earl L
AU  - Gray EL
FAU - Wolf, Cynthia
AU  - Wolf C
FAU - Needham, Larry L
AU  - Needham LL
FAU - Calafat, Antonia M
AU  - Calafat AM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Phthalic Acids)
RN  - 0 (Plasticizers)
RN  - 8X3RJ0527W (di-n-octyl phthalate)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Female
MH  - Inactivation, Metabolic
MH  - Mass Spectrometry
MH  - Microsomes, Liver/*metabolism
MH  - Oxidation-Reduction
MH  - Phthalic Acids/metabolism/*urine
MH  - Plasticizers/*pharmacokinetics
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2005/04/21 09:00
MHDA- 2005/06/02 09:00
CRDT- 2005/04/21 09:00
PHST- 2004/10/29 00:00 [received]
PHST- 2005/01/20 00:00 [revised]
PHST- 2005/01/21 00:00 [accepted]
PHST- 2005/04/21 09:00 [pubmed]
PHST- 2005/06/02 09:00 [medline]
PHST- 2005/04/21 09:00 [entrez]
AID - S0300-483X(05)00071-5 [pii]
AID - 10.1016/j.tox.2005.01.012 [doi]
PST - ppublish
SO  - Toxicology. 2005 Jun 1;210(2-3):123-33. doi: 10.1016/j.tox.2005.01.012.