PMID- 15841156 OWN - NLM STAT- MEDLINE DCOM- 20061212 LR - 20171116 IS - 1671-167X (Print) IS - 1671-167X (Linking) VI - 37 IP - 2 DP - 2005 Apr 18 TI - [High performance liquid chromatogram (HPLC) determination of adenosine phosphates in rat myocardium]. PG - 201-2 AB - OBJECTIVE: To establish method for the quantitative determination of adenosine phosphates in rat myocardium by optimized high performance liquid chromatogram (HPLC). METHODS: ODS HYPERSIL C(18) column and a mobile phase of 50 mmol/L tribasic potassium phosphate buffer solution (pH 6.5), with UV detector at 254 nm were used. RESULTS: The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively; relative standard deviations (RSDs) of within-day and between-days were less than 1.5% and 5.1%, respectively. CONCLUSION: The method is simple, rapid and accurate, and can be used to analyse the adenosine phosphates in myocardium. FAU - Miao, Yu AU - Miao Y AD - Department of Cardiology, Xi yuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China. miaoyu0609@sina.com FAU - Wang, Cheng-long AU - Wang CL FAU - Yin, Hui-jun AU - Yin HJ FAU - Shi, Da-zhuo AU - Shi DZ FAU - Chen, Ke-ji AU - Chen KJ LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Beijing Da Xue Xue Bao Yi Xue Ban JT - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences JID - 101125284 RN - 0 (Adenine Nucleotides) RN - 415SHH325A (Adenosine Monophosphate) RN - 61D2G4IYVH (Adenosine Diphosphate) RN - 8L70Q75FXE (Adenosine Triphosphate) SB - IM MH - Adenine Nucleotides/analysis MH - Adenosine Diphosphate/*analysis MH - Adenosine Monophosphate/*analysis MH - Adenosine Triphosphate/*analysis MH - Animals MH - Chromatography, High Pressure Liquid MH - Male MH - Myocardium/*chemistry MH - Rats MH - Rats, Wistar EDAT- 2005/04/21 09:00 MHDA- 2006/12/13 09:00 CRDT- 2005/04/21 09:00 PHST- 2005/04/21 09:00 [pubmed] PHST- 2006/12/13 09:00 [medline] PHST- 2005/04/21 09:00 [entrez] PST - ppublish SO - Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Apr 18;37(2):201-2.