PMID- 15842664
OWN - NLM
STAT- MEDLINE
DCOM- 20050614
LR  - 20161124
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 129
IP  - 3
DP  - 2005 May
TI  - UTY-specific TCR-transfer generates potential graft-versus-leukaemia effector T 
      cells.
PG  - 392-402
AB  - Immunotherapeutic approaches that target antigens that are differentially 
      recognized on haematopoietic and non-haematopoietic cells may specifically 
      enhance the graft-versus-leukaemia (GVL) effect of donor lymphocyte infusion. In 
      this study, we have characterized a new HLA-B*5201-restricted epitope of the UTY 
      gene. Unusually, presentation of this epitope was restricted to lymphoblasts. As 
      a result, a T cell clone specific to this epitope recognized normal and malignant 
      male B and T lymphoblasts, while showing little reactivity towards male 
      HLA-B*5201+ fibroblasts. Transfer of its T cell receptor (TCR) into donor T cells 
      led to the generation of large numbers of T cells, which acquired the specificity 
      of the original clone, its avidity and the differential pattern of reactivity 
      towards lymphoblasts and fibroblasts. Remarkably, the specific response of 
      TCR-transferred T cells was significantly higher than that of the original clone. 
      This is the first demonstration of the possibility to preserve the specific 
      pattern of a T cell response to a differentially expressed antigen after 
      TCR-transfer and to augment the amplitude of this response concomitantly. These 
      results indicate that it may be feasible to enhance the GVL effect of donor 
      lymphocyte infusions in lymphoproliferative malignancies by the transfer of TCRs 
      specific to epitopes that are differentially recognized on lymphoblasts.
FAU - Ivanov, Roman
AU  - Ivanov R
AD  - Jordan Laboratory for Haemato-Oncology, Department of Haematology, University 
      Medical Centre Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands.
FAU - Hol, Samantha
AU  - Hol S
FAU - Aarts, Tineke
AU  - Aarts T
FAU - Hagenbeek, Anton
AU  - Hagenbeek A
FAU - Slager, Elisabeth H
AU  - Slager EH
FAU - Ebeling, Saskia
AU  - Ebeling S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (UTY protein, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Proliferation
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Feasibility Studies
MH  - Graft vs Leukemia Effect/*immunology
MH  - HLA-B Antigens/genetics/immunology
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - *Lymphocyte Transfusion
MH  - Male
MH  - Minor Histocompatibility Antigens
MH  - Nuclear Proteins
MH  - Peptide Fragments/immunology
MH  - Proteins/*immunology
MH  - Receptors, Antigen, T-Cell/*immunology
MH  - Retroviridae/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Transfection
EDAT- 2005/04/22 09:00
MHDA- 2005/06/15 09:00
CRDT- 2005/04/22 09:00
PHST- 2005/04/22 09:00 [pubmed]
PHST- 2005/06/15 09:00 [medline]
PHST- 2005/04/22 09:00 [entrez]
AID - BJH5461 [pii]
AID - 10.1111/j.1365-2141.2005.05461.x [doi]
PST - ppublish
SO  - Br J Haematol. 2005 May;129(3):392-402. doi: 10.1111/j.1365-2141.2005.05461.x.