PMID- 15845255
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20171116
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 110
IP  - 1-2
DP  - 2005 Jun
TI  - Co-purification of soluble membrane cofactor protein (CD46) and human herpesvirus 
      6 variant A genome in serum from multiple sclerosis patients.
PG  - 57-63
AB  - The association of human herpesvirus 6 (HHV-6) and multiple sclerosis (MS) has 
      been supported by several immunological and molecular studies. Recently, membrane 
      cofactor protein (CD46) has been identified as the cellular receptor for the A 
      and B variants of HHV-6. Elevated levels of soluble CD46 (sCD46) have been 
      reported in the serum and CSF of MS patients. The aim of this study was to 
      investigate a possible correlation between elevated levels of soluble CD46 and 
      the presence of serum HHV-6 DNA in MS patients. An immunoaffinity column 
      comprised of immobilized monoclonal antibodies to CD46 was developed to isolate 
      sCD46 from cell free body fluids of MS patients and controls. After 
      immunoaffinity purification, DNA was extracted from anti-CD46 column eluates and 
      subjected to PCR amplification. Of the 42 MS samples tested, 4 serum samples were 
      HHV-6 positive, 3 of which were typed as HHV-6A. The co-purification of sCD46 and 
      HHV-6 DNA from MS sera indicates that HHV-6 is tightly connected to its receptor, 
      CD46, in the serum of MS patients.
FAU - Fogdell-Hahn, A
AU  - Fogdell-Hahn A
AD  - Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke 
      (NINDS), National Institutes of Health (NIH), 9000 Rockville Pike, Building 10, 
      Room 5B-16, Bethesda, MD 20892, USA.
FAU - Soldan, S S
AU  - Soldan SS
FAU - Shue, S
AU  - Shue S
FAU - Akhyani, N
AU  - Akhyani N
FAU - Refai, H
AU  - Refai H
FAU - Ahlqvist, J
AU  - Ahlqvist J
FAU - Jacobson, S
AU  - Jacobson S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antigens, CD)
RN  - 0 (CD46 protein, human)
RN  - 0 (DNA, Viral)
RN  - 0 (Membrane Cofactor Protein)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Antigens, CD/blood/*isolation & purification
MH  - Chromatography, Affinity
MH  - DNA, Viral/analysis/isolation & purification
MH  - Female
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Humans
MH  - Male
MH  - Membrane Cofactor Protein
MH  - Membrane Glycoproteins/blood/*isolation & purification
MH  - Multiple Sclerosis/*virology
MH  - Polymerase Chain Reaction
MH  - Serum/virology
EDAT- 2005/04/23 09:00
MHDA- 2005/10/01 09:00
CRDT- 2005/04/23 09:00
PHST- 2004/10/21 00:00 [received]
PHST- 2004/12/21 00:00 [revised]
PHST- 2005/01/07 00:00 [accepted]
PHST- 2005/04/23 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2005/04/23 09:00 [entrez]
AID - S0168-1702(05)00023-7 [pii]
AID - 10.1016/j.virusres.2005.01.005 [doi]
PST - ppublish
SO  - Virus Res. 2005 Jun;110(1-2):57-63. doi: 10.1016/j.virusres.2005.01.005.