PMID- 15845453
OWN - NLM
STAT- MEDLINE
DCOM- 20050531
LR  - 20071115
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 22
IP  - 4
DP  - 2005 Apr
TI  - CCL19 and CCL21 induce a potent proinflammatory differentiation program in 
      licensed dendritic cells.
PG  - 493-505
AB  - Dendritic cells (DCs) are key instigators of adaptive immune responses. Using an 
      alphaviral expression cloning technology, we have identified the chemokine CCL19 
      as a potent inducer of T cell proliferation in a DC-T cell coculture system. 
      Subsequent studies showed that CCL19 enhanced T cell proliferation by inducing 
      maturation of DCs, resulting in upregulation of costimulatory molecules and the 
      production of proinflammatory cytokines. Moreover, CCL19 programmed DCs for the 
      induction of T helper type (Th) 1 rather than Th2 responses. Importantly, only 
      activated DCs that migrated from the periphery to draining lymph nodes, but not 
      resting steady-state DCs residing within lymph nodes, expressed high levels of 
      CCR7 in vivo and responded to CCL19 with the production of proinflammatory 
      cytokines. Migrating DCs isolated from mice genetically deficient in CCL19 and 
      CCL21 (plt/plt) presented an only partially mature phenotype, highlighting the 
      importance of these chemokines for full DC maturation in vivo. Our findings 
      indicate that CCL19 and CCL21 are potent natural adjuvants for terminal 
      activation of DCs and suggest that chemokines not only orchestrate DC migration 
      but also regulate their immunogenic potential for the induction of T cell 
      responses.
FAU - Marsland, Benjamin J
AU  - Marsland BJ
AD  - Molecular Biomedicine, Department of Environmental Sciences, Swiss Federal 
      Institute of Technology, CH-8092 Zurich, Switzerland.
FAU - Battig, Patrick
AU  - Battig P
FAU - Bauer, Monika
AU  - Bauer M
FAU - Ruedl, Christiane
AU  - Ruedl C
FAU - Lassing, Ute
AU  - Lassing U
FAU - Beerli, Roger R
AU  - Beerli RR
FAU - Dietmeier, Klaus
AU  - Dietmeier K
FAU - Ivanova, Lidia
AU  - Ivanova L
FAU - Pfister, Thomas
AU  - Pfister T
FAU - Vogt, Lorenz
AU  - Vogt L
FAU - Nakano, Hideki
AU  - Nakano H
FAU - Nembrini, Chiara
AU  - Nembrini C
FAU - Saudan, Philippe
AU  - Saudan P
FAU - Kopf, Manfred
AU  - Kopf M
FAU - Bachmann, Martin F
AU  - Bachmann MF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Ccl19 protein, mouse)
RN  - 0 (Ccl21c protein, mouse)
RN  - 0 (Chemokine CCL19)
RN  - 0 (Chemokine CCL21)
RN  - 0 (Chemokines, CC)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/immunology
MH  - Chemokine CCL19
MH  - Chemokine CCL21
MH  - Chemokines, CC/genetics/isolation & purification/*physiology
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*cytology/immunology/metabolism
MH  - Gene Expression
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - Receptors, Cell Surface/metabolism
MH  - T-Lymphocytes/immunology
MH  - Th1 Cells/immunology
MH  - Toll-Like Receptors
MH  - Up-Regulation
EDAT- 2005/04/23 09:00
MHDA- 2005/06/01 09:00
CRDT- 2005/04/23 09:00
PHST- 2004/11/08 00:00 [received]
PHST- 2005/02/10 00:00 [revised]
PHST- 2005/02/23 00:00 [accepted]
PHST- 2005/04/23 09:00 [pubmed]
PHST- 2005/06/01 09:00 [medline]
PHST- 2005/04/23 09:00 [entrez]
AID - S1074-7613(05)00097-X [pii]
AID - 10.1016/j.immuni.2005.02.010 [doi]
PST - ppublish
SO  - Immunity. 2005 Apr;22(4):493-505. doi: 10.1016/j.immuni.2005.02.010.