PMID- 15845594
OWN - NLM
STAT- MEDLINE
DCOM- 20050927
LR  - 20050718
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 20
IP  - 8
DP  - 2005 Aug
TI  - Frequency of aneuploidy in sperm from patients with extremely severe male factor 
      infertility.
PG  - 2140-52
AB  - BACKGROUND: A protocol for the chromosomal analysis of sperm samples with a 
      severely reduced number of sperm cells was designed. METHODS: A severe male 
      factor condition was the main cause of infertility for 38 couples: 27 were 
      oligoasthenoteratospermic (OAT) and 11 with non-obstructive azoospermia underwent 
      testicular sperm extraction (TESE). A two-round fluorescence in situ 
      hybridization (FISH) protocol was performed with probes specific for the 
      chromosomes X, Y, 13, 15, 16, 17, 18, 21 and 22. The recording of the position of 
      each sperm cell at the microscope allowed diagnosis of each spermatozoon for the 
      nine tested chromosomes. RESULTS: A mean number of 122+/-78.5 sperm were 
      diagnosed per patient with an incidence of total abnormalities corresponding to 
      13.4%. chi2-tests for the observed frequencies and goodness-of-fit test were 
      highly significant in all cases. A significantly higher proportion of total 
      aneuploidy was detected in 79% of the tested samples compared to the normal 
      population. Testicular sperm were significantly more prone to aneuploidy than 
      ejaculated sperm. CONCLUSIONS: The designed FISH protocol for the analysis of 
      severe OAT and TESE sperm samples is reliable, implying that the studied sample 
      is representative of the original population. In view of the high incidence of 
      aneuploidy in most severe OAT and TESE sperm, the FISH analysis of pathological 
      sperm samples can be routinely performed in order to estimate the chances of the 
      paternal contribution to aneuploidy in the resulting embryos.
FAU - Gianaroli, Luca
AU  - Gianaroli L
AD  - S.I.S.Me.R. Reproductive Medicine Unit, via Mazzini 12, 40138 Bologna, Dept 
      Ob/Gyn, S. Martino Hospital, University of Genoa, Largo Rosanna Benzi 10, 16132 
      Genoa, Italy. sismer@sismer.it
FAU - Magli, M Cristina
AU  - Magli MC
FAU - Cavallini, Giorgio
AU  - Cavallini G
FAU - Crippa, Andor
AU  - Crippa A
FAU - Nadalini, Marco
AU  - Nadalini M
FAU - Bernardini, Luca
AU  - Bernardini L
FAU - Menchini Fabris, Giuseppe F
AU  - Menchini Fabris GF
FAU - Voliani, Silvia
AU  - Voliani S
FAU - Ferraretti, Anna P
AU  - Ferraretti AP
LA  - eng
PT  - Journal Article
DEP - 20050421
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - Male
MH  - Middle Aged
MH  - Oligospermia/*diagnosis/epidemiology/*genetics
MH  - Preimplantation Diagnosis/*methods/standards
MH  - Reproducibility of Results
MH  - Reproductive Techniques, Assisted
MH  - *Spermatozoa
EDAT- 2005/04/23 09:00
MHDA- 2005/09/28 09:00
CRDT- 2005/04/23 09:00
PHST- 2005/04/23 09:00 [pubmed]
PHST- 2005/09/28 09:00 [medline]
PHST- 2005/04/23 09:00 [entrez]
AID - dei033 [pii]
AID - 10.1093/humrep/dei033 [doi]
PST - ppublish
SO  - Hum Reprod. 2005 Aug;20(8):2140-52. doi: 10.1093/humrep/dei033. Epub 2005 Apr 21.