PMID- 15847646
OWN - NLM
STAT- MEDLINE
DCOM- 20050922
LR  - 20050425
IS  - 0001-6772 (Print)
IS  - 0001-6772 (Linking)
VI  - 184
IP  - 1
DP  - 2005 May
TI  - Abnormal renal structural alterations during the development of diabetes mellitus 
      in Otsuka Long-Evans Tokushima Fatty rats.
PG  - 73-81
AB  - AIM: The aim of this study was to investigate the renal structural properties in 
      diabetic nephropathy. METHODS: Flow-pressure and pressure-glomerular filtration 
      rate (GFR) relationships were determined for maximally vasodilated kidneys at 10 
      (pre-diabetic stage) and 42 weeks of age (diabetic stage) in Otsuka Long-Evans 
      Tokushima Fatty rats (OLETF), an animal model of type 2 diabetes mellitus, using 
      age-matched Long-Evans Tokushima Otsuka rats (LETO) as non-diabetic controls (n = 
      9 of each age for each strain). Kidneys were then perfusion-fixed for 
      histological analysis. RESULTS: At 10 weeks of age, the slope of flow-pressure 
      relationship (minimal renal vascular resistance, reflecting overall luminal 
      dimensions of preglomerular and postglomerular vasculature) was steeper in OLETF 
      than in LETO. In contrast, the threshold pressure for beginning filtration 
      (preglomerular-to-postglomerular vascular resistance ratio) at pressure-GFR 
      relationship did not differ between the two strains; however, the slope of the 
      relationship (glomerular filtration capacity) was lower in OLETF than in LETO. 
      Thus, in the kidneys of 10-week-old OLETF rats, vascular narrowing and impaired 
      glomerular filtration capacity already existed with no abnormalities in 
      preglomerular-to-postglomerular vascular resistance ratio. From the age of 10-42 
      weeks, the following results were obtained: (1) Minimal renal vascular resistance 
      decreased in both strains, but it diminished markedly in OLETF. (2) The pressure 
      for beginning filtration increased in LETO, but remained unchanged in OLETF. (3) 
      Glomerular filtration capacity decreased to the similar extent in both strains. 
      (4) Histologically, the vascular lumen and wall thickness increased in the 
      interlobular arteries of both strains. However, vascular luminal widening was 
      more pronounced in OLETF, resulting in the reduction in wall to lumen ratio. (5) 
      Glomerular injuries and increased blood pressure occurred only in OLETF. 
      CONCLUSION: In conclusion, during progression from the prediabetic to diabetic 
      stage of OLETF, the pre-existing vascular narrowing was markedly attenuated 
      without the concomitant increase in preglomerular-to-postglomerular vascular 
      resistance ratio. Combined with increased blood pressure, these renal structural 
      alterations could lead to the elevation of intraglomerular pressure in OLETF.
FAU - Koike, T
AU  - Koike T
AD  - The Second Department of Internal Medicine, Toyama Medical and Pharmaceutical 
      University, Sugitani, Toyama, Japan.
FAU - Tomoda, F
AU  - Tomoda F
FAU - Kinuno, H
AU  - Kinuno H
FAU - Inoue, H
AU  - Inoue H
FAU - Takata, M
AU  - Takata M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Acta Physiol Scand
JT  - Acta physiologica Scandinavica
JID - 0370362
RN  - 0 (Blood Glucose)
SB  - IM
MH  - Animals
MH  - Blood Glucose/analysis
MH  - Blood Pressure/physiology
MH  - Diabetes Mellitus, Type 2/*pathology/physiopathology
MH  - Diabetic Nephropathies/*pathology/physiopathology
MH  - Glomerular Filtration Rate/physiology
MH  - Kidney/*blood supply/pathology/physiopathology
MH  - Male
MH  - Organ Size/physiology
MH  - Proteinuria/complications
MH  - Rats
MH  - Rats, Inbred OLETF
MH  - Vascular Resistance/physiology
MH  - Vasodilation/physiology
EDAT- 2005/04/26 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/04/26 09:00
PHST- 2005/04/26 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/04/26 09:00 [entrez]
AID - APS1418 [pii]
AID - 10.1111/j.1365-201X.2005.01418.x [doi]
PST - ppublish
SO  - Acta Physiol Scand. 2005 May;184(1):73-81. doi: 10.1111/j.1365-201X.2005.01418.x.