PMID- 15849799
OWN - NLM
STAT- MEDLINE
DCOM- 20050726
LR  - 20050502
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 25
IP  - 4
DP  - 2005 Apr
TI  - Fluorescent in situ hybridization (FISH) on paraffin-embedded placental tissues 
      as an adjunct for understanding the etiology of early spontaneous abortion.
PG  - 314-7
AB  - OBJECTIVES: An investigation of first-trimester spontaneous abortions (SAs) for 
      those cases in which karyotype is not available was designed to test the 
      efficiency of fluorescence in situ hybridization (FISH) on paraffin-embedded 
      tissues combined with pathological examination for understanding the etiology of 
      SAs. METHODS: Pathological examination of 202 placental tissues from SAs was 
      performed. FISH analysis was then carried out on paraffin-embedded tissue 
      sections from the same abortion products with probes specific for chromosomes 13, 
      16, 18, 21, X, Y. RESULTS: FISH could be achieved in 196 cases (97%). After 
      pathological analysis alone, the etiology of SAs was evoked in 40 cases. The 
      suspected diagnosis was confirmed by FISH in 26 cases (13.2%). After combined 
      pathological and FISH analysis, the etiology of SAs was identified in 83 from the 
      196 cases (42.3%) with the probe set used. CONCLUSION: The present study 
      demonstrates the value of FISH on paraffin-embedded tissues as an adjunct for 
      understanding the etiology of SAs for those cases in which karyotype is not 
      available. Combination of pathological and FISH analysis increases the yield of 
      diagnosis by a factor of 3.2. The results also demonstrate that predictions of 
      the karyotype from pathological examination should be avoided.
FAU - Lescoat, D
AU  - Lescoat D
AD  - Histologie, Embryologie et Cytogenetique, Faculte de Medecine, 35043 Rennes, 
      France. denise.lescoat@univ-rennes1.fr
FAU - Jouan, H
AU  - Jouan H
FAU - Loeuillet-Olivo, L
AU  - Loeuillet-Olivo L
FAU - Le Calve, M
AU  - Le Calve M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Abortion, Spontaneous/*genetics/pathology
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding
MH  - Placenta/*pathology
MH  - Pregnancy
MH  - *Pregnancy Trimester, First
EDAT- 2005/04/26 09:00
MHDA- 2005/07/27 09:00
CRDT- 2005/04/26 09:00
PHST- 2005/04/26 09:00 [pubmed]
PHST- 2005/07/27 09:00 [medline]
PHST- 2005/04/26 09:00 [entrez]
AID - 10.1002/pd.1132 [doi]
PST - ppublish
SO  - Prenat Diagn. 2005 Apr;25(4):314-7. doi: 10.1002/pd.1132.