PMID- 15851598
OWN - NLM
STAT- MEDLINE
DCOM- 20051227
LR  - 20220409
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 111
IP  - 17
DP  - 2005 May 3
TI  - Monitoring of bone marrow cell homing into the infarcted human myocardium.
PG  - 2198-202
AB  - BACKGROUND: Intracoronary transfer of autologous bone marrow cells (BMCs) 
      promotes recovery of left ventricular systolic function in patients with acute 
      myocardial infarction. Although the mechanisms of this effect remain to be 
      established, homing of BMCs into the infarcted myocardium is probably a critical 
      early event. METHODS AND RESULTS: We determined BMC biodistribution after 
      therapeutic application in patients with a first ST-segment-elevation myocardial 
      infarction who had undergone stenting of the infarct-related artery. Unselected 
      BMCs were radiolabeled with 100 MBq 2-[18F]-fluoro-2-deoxy-D-glucose (18F-FDG) 
      and infused into the infarct-related coronary artery (intracoronary; n=3 
      patients) or injected via an antecubital vein (intravenous; n=3 patients). In 3 
      additional patients, CD34-positive (CD34+) cells were immunomagnetically enriched 
      from unselected BMCs, labeled with 18F-FDG, and infused intracoronarily. Cell 
      transfer was performed 5 to 10 days after stenting. More than 99% of the infused 
      total radioactivity was cell bound. Nucleated cell viability, comparable in all 
      preparations, ranged from 92% to 96%. Fifty to 75 minutes after cell transfer, 
      all patients underwent 3D PET imaging. After intracoronary transfer, 1.3% to 2.6% 
      of 18F-FDG-labeled unselected BMCs were detected in the infarcted myocardium; the 
      remaining activity was found primarily in liver and spleen. After intravenous 
      transfer, only background activity was detected in the infarcted myocardium. 
      After intracoronary transfer of 18F-FDG-labeled CD34-enriched cells, 14% to 39% 
      of the total activity was detected in the infarcted myocardium. Unselected BMCs 
      engrafted in the infarct center and border zone; homing of CD34-enriched cells 
      was more pronounced in the border zone. CONCLUSIONS: 18F-FDG labeling and 3D PET 
      imaging can be used to monitor myocardial homing and biodistribution of BMCs 
      after therapeutic application in patients.
FAU - Hofmann, Michael
AU  - Hofmann M
AD  - Department of Nuclear Medicine, Hanover Medical School, Hanover, Germany.
FAU - Wollert, Kai C
AU  - Wollert KC
FAU - Meyer, Gerd P
AU  - Meyer GP
FAU - Menke, Alix
AU  - Menke A
FAU - Arseniev, Lubomir
AU  - Arseniev L
FAU - Hertenstein, Bernd
AU  - Hertenstein B
FAU - Ganser, Arnold
AU  - Ganser A
FAU - Knapp, Wolfram H
AU  - Knapp WH
FAU - Drexler, Helmut
AU  - Drexler H
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050425
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Antigens, CD34)
RN  - 0Z5B2CJX4D (Fluorodeoxyglucose F18)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD34
MH  - Bone Marrow Cells/*physiology
MH  - Bone Marrow Transplantation/*methods
MH  - *Cell Movement
MH  - Fluorodeoxyglucose F18
MH  - Humans
MH  - Leukocytes
MH  - Male
MH  - Middle Aged
MH  - Myocardial Infarction/*therapy
MH  - Myocardium/cytology
MH  - Organ Specificity
MH  - Positron-Emission Tomography
MH  - Transplantation, Autologous
MH  - Ventricular Dysfunction, Left/*therapy
EDAT- 2005/04/27 09:00
MHDA- 2005/12/28 09:00
CRDT- 2005/04/27 09:00
PHST- 2005/04/27 09:00 [pubmed]
PHST- 2005/12/28 09:00 [medline]
PHST- 2005/04/27 09:00 [entrez]
AID - 01.CIR.0000163546.27639.AA [pii]
AID - 10.1161/01.CIR.0000163546.27639.AA [doi]
PST - ppublish
SO  - Circulation. 2005 May 3;111(17):2198-202. doi: 
      10.1161/01.CIR.0000163546.27639.AA. Epub 2005 Apr 25.