PMID- 15852352
OWN - NLM
STAT- MEDLINE
DCOM- 20050922
LR  - 20131121
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 26
IP  - 11
DP  - 2005 Jun
TI  - On-line concentration of peptides and proteins with the hyphenation of polymer 
      monolithic immobilized metal affinity chromatography and capillary 
      electrophoresis.
PG  - 2172-8
AB  - An iminodiacetic acid (IDA)-type adsorbent is prepared at the one end of a 
      capillary by covalently bonding IDA to the monolithic rods of macroporous 
      poly(glycidyl methacrylate-co-ethylene dimethacrylate). Cu(II) is later 
      introduced to the support via the interaction with IDA. By this means, polymer 
      monolithic immobilized metal affinity chromatography (IMAC) materials are 
      prepared. With such a column, IMAC for on-line concentration and capillary 
      electrophoresis (CE) for the subsequent analysis are hyphenated for the analysis 
      of peptides and proteins. The reproducibility of such a column has been proved 
      good with relative standard deviations (RSDs) of dead time of less than 5% for 
      injection-to-injection and 12% for column-to-column (n = 3). Through application 
      on the analysis of standard peptides and real protein samples, such a technique 
      has shown promising in proteome study.
FAU - Zhang, Lingyi
AU  - Zhang L
AD  - National Chromatographic Research & Analysis Center, Dalian Institute of Chemical 
      Physics, Chinese Academy of Sciences, Dalian, PR China.
FAU - Zhang, Lihua
AU  - Zhang L
FAU - Zhang, Weibing
AU  - Zhang W
FAU - Zhang, Yukui
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Imino Acids)
RN  - 0 (Metals)
RN  - 0 (Methylmethacrylates)
RN  - 0 (Peptides)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 31743-77-8 (GMA-EDMA)
RN  - 789U1901C5 (Copper)
RN  - XQM2L81M8Z (iminodiacetic acid)
SB  - IM
MH  - Chromatography, Affinity/*methods
MH  - Copper
MH  - Electrophoresis, Capillary/*methods
MH  - Imino Acids
MH  - Metals
MH  - Methylmethacrylates
MH  - Online Systems
MH  - Peptides/*analysis
MH  - Polymers
MH  - Porosity
MH  - Proteins/*analysis
MH  - Proteomics/methods
EDAT- 2005/04/27 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/04/27 09:00
PHST- 2005/04/27 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/04/27 09:00 [entrez]
AID - 10.1002/elps.200410377 [doi]
PST - ppublish
SO  - Electrophoresis. 2005 Jun;26(11):2172-8. doi: 10.1002/elps.200410377.