PMID- 15852499
OWN - NLM
STAT- MEDLINE
DCOM- 20050811
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 206
IP  - 3
DP  - 2005 Jul
TI  - E-cadherin-dependent adhesion of dendritic and Langerhans cells to keratinocytes 
      is defective in cervical human papillomavirus-associated (pre)neoplastic lesions.
PG  - 346-55
AB  - Although human papillomavirus (HPV) DNA is detected in the majority of squamous 
      intraepithelial lesions (SILs) and squamous cell carcinomas (SCCs) of the uterine 
      cervix, the persistence and progression of cervical lesions suggest that viral 
      antigens are not adequately presented to the immune system. This hypothesis is 
      reinforced by the observation that most SILs show quantitative and functional 
      alterations of Langerhans cells (LCs). The aim of this study was to determine 
      whether modulation of E-cadherin-mediated homophilic and heterotypic interactions 
      between keratinocytes and LCs is involved in these abnormalities of LCs in 
      (pre)neoplastic cervical epithelium. Cell membrane expression of E-cadherin and 
      the density of CD1a+ LCs were low in the epithelium of SILs and SCC biopsy 
      specimens, compared with normal exocervical epithelium. Dendritic cells (DCs) and 
      LCs generated in vitro were randomly distributed throughout the full thickness of 
      organotypic cultures of E-cadherin- HPV-transformed cells. In contrast, these 
      cells rapidly adhered to the keratinocyte cell layers when HPV-transformed cells 
      transfected with E-cadherin were used. These data suggest that the 
      E-cadherin-mediated contact between keratinocytes and LCs is potentially 
      important for initiating or maintaining the immune response during chronic HPV 
      infection.
CI  - Copyright 2005 Pathological Society of Great Britain and Ireland. Published by 
      John Wiley & Sons, Ltd.
FAU - Hubert, Pascale
AU  - Hubert P
AD  - Department of Pathology, CRCE, B35, CHU Sart Tilman, 4000 Liege, Belgium.
FAU - Caberg, Jean-Hubert
AU  - Caberg JH
FAU - Gilles, Christine
AU  - Gilles C
FAU - Bousarghin, Latifa
AU  - Bousarghin L
FAU - Franzen-Detrooz, Elizabeth
AU  - Franzen-Detrooz E
FAU - Boniver, Jacques
AU  - Boniver J
FAU - Delvenne, Philippe
AU  - Delvenne P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
RN  - 0 (Cadherins)
SB  - IM
MH  - Antigens, CD1/immunology
MH  - Cadherins/*analysis
MH  - Carcinoma in Situ/genetics/physiopathology/virology
MH  - Carcinoma, Squamous Cell/genetics/physiopathology/virology
MH  - Cell Adhesion/physiology
MH  - Cell Membrane/pathology
MH  - Dendritic Cells/pathology/*physiology
MH  - Epithelial Cells/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Keratinocytes/pathology/*physiology
MH  - Langerhans Cells/pathology/*physiology
MH  - Papillomavirus Infections/genetics/*physiopathology
MH  - Phenotype
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/genetics/*physiopathology/virology
EDAT- 2005/04/27 09:00
MHDA- 2005/08/12 09:00
CRDT- 2005/04/27 09:00
PHST- 2005/04/27 09:00 [pubmed]
PHST- 2005/08/12 09:00 [medline]
PHST- 2005/04/27 09:00 [entrez]
AID - 10.1002/path.1771 [doi]
PST - ppublish
SO  - J Pathol. 2005 Jul;206(3):346-55. doi: 10.1002/path.1771.