PMID- 15854219
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20220331
IS  - 0160-564X (Print)
IS  - 0160-564X (Linking)
VI  - 29
IP  - 5
DP  - 2005 May
TI  - Characterization of a hollow fiber bioartificial liver device.
PG  - 419-22
AB  - A three-compartment bioartificial liver (BAL) has been developed for potential 
      treatment of fulminant hepatic failure. It has been shown previously that 
      viability and liver-specific functions were maintained in laboratory-scale 
      bioreactors of such design. In this study, the performance of hepatocytes in a 
      clinical-scale bioartificial liver was verified by sustained specific production 
      rates of albumin and urea, along with oxygen consumption rates for up to 56 h and 
      liver-specific gene expression for up to 72 h. In addition, transmission of 
      porcine endogenous retrovirus and other type C retroviral particles across the 
      hollow fibers was not detected under both normal and extreme operating fluxes. 
      These results demonstrate that the clinical-scale BAL performs at a level similar 
      to the laboratory scale and that it offers a viral barrier against porcine 
      retroviruses.
FAU - Abu-Absi, Susan Fugett
AU  - Abu-Absi SF
AD  - Department of Chemical Engineering and Materials Science, University of 
      Minnesota, Minneapolis, MN 55455-0132, U.S.A.
FAU - Seth, Gargi
AU  - Seth G
FAU - Narayanan, Ramanathan A
AU  - Narayanan RA
FAU - Groehler, Kristine
AU  - Groehler K
FAU - Lai, Pamela
AU  - Lai P
FAU - Anderson, Mary L
AU  - Anderson ML
FAU - Sielaff, Timothy
AU  - Sielaff T
FAU - Hu, Wei-Shou
AU  - Hu WS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (Albumins)
RN  - 63231-63-0 (RNA)
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - Albumins/metabolism
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Hepatocytes/cytology/*metabolism
MH  - Liver Failure, Acute/therapy
MH  - Liver, Artificial/*standards
MH  - RNA/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
MH  - Urea/metabolism
EDAT- 2005/04/28 09:00
MHDA- 2006/01/06 09:00
CRDT- 2005/04/28 09:00
PHST- 2005/04/28 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/04/28 09:00 [entrez]
AID - AOR29070 [pii]
AID - 10.1111/j.1525-1594.2005.29070.x [doi]
PST - ppublish
SO  - Artif Organs. 2005 May;29(5):419-22. doi: 10.1111/j.1525-1594.2005.29070.x.