PMID- 15854409
OWN - NLM
STAT- MEDLINE
DCOM- 20061222
LR  - 20081121
IS  - 1001-0939 (Print)
IS  - 1001-0939 (Linking)
VI  - 28
IP  - 3
DP  - 2005 Mar
TI  - [A study of interferon-gamma induced airway mucous cell apoptosis and its 
      mechanisms].
PG  - 160-3
AB  - OBJECTIVE: To investigate the effect of interferon-gamma (IFN-gamma) on airway 
      mucous cells and its mechanisms. METHODS: (1) Normal human bronchial epithelial 
      cells (NHBEs) were cultured under specific conditions, and treated by IFN-gamma 
      for 3 days. The cells were analyzed with fluorescein isothiocyanate (FITC) and 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay. (2) Twenty wild type C57BL/6J mice were immunized and divided into 4 
      groups, and treated with IFN-gamma (50 ng and 100 ng, respectively), 
      interleukin-13 (IL-13, 5 microg) and saline by nostril instillation. The mice 
      were sacrificed and the airway mucous cells were analyzed by morphometry and 
      TUNEL assay. RESULTS: (1) IFN-gamma induced apoptosis in NHBEs, which showed 
      condensed nuclei, nuclear and DNA fragmentaion, and were positive by TUNEL assay. 
      Bax was upregulated and translocated from cell plasma to mitochondria under the 
      treatment. (2) Airway mucous cell account in 100 ng IFN-gamma instillation 
      immunized mice group (28 +/- 6 mucous cells/mm basal lamina) was significantly 
      decreased as compared to that in saline (58 +/- 12) and IL-13 (59 +/- 6) 
      instillation groups (all < 0.05). There was no difference among the IFN-gamma 50 
      ng (48 +/- 11), saline (58 +/- 12) and IL-13 (59 +/- 6) instillation groups (all 
      P > 0.05). TUNEL assay was also positive in airway mucous cells from IFN-gamma 
      instillation mice as compared to saline instillation mice. CONCLUSIONS: IFN-gamma 
      leads to airway mucous cell apoptosis by Bax upregulating and translocation into 
      mitochondria. This might be of significance in the new therapies of asthma.
FAU - Shi, Zhao-quan
AU  - Shi ZQ
AD  - Department of Respiratory Medicine, Second Affiliated Hospital of the Second 
      Military Medical University, Shanghai 200003, China.
FAU - Feng, Yan
AU  - Feng Y
FAU - Hou, Yuan-kai
AU  - Hou YK
FAU - Liu, Tao
AU  - Liu T
FAU - Xiu, Qing-yu
AU  - Xiu QY
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Jie He He Hu Xi Za Zhi
JT  - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal 
      of tuberculosis and respiratory diseases
JID - 8712226
RN  - 0 (bcl-2-Associated X Protein)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Asthma/metabolism/*pathology
MH  - Bronchi/cytology/pathology
MH  - Cell Count
MH  - Cells, Cultured
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Respiratory Mucosa/drug effects/*pathology
MH  - Up-Regulation/drug effects
MH  - bcl-2-Associated X Protein/biosynthesis/genetics
EDAT- 2005/04/28 09:00
MHDA- 2006/12/23 09:00
CRDT- 2005/04/28 09:00
PHST- 2005/04/28 09:00 [pubmed]
PHST- 2006/12/23 09:00 [medline]
PHST- 2005/04/28 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Jie He He Hu Xi Za Zhi. 2005 Mar;28(3):160-3.