PMID- 15854584
OWN - NLM
STAT- MEDLINE
DCOM- 20050715
LR  - 20131121
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1042
IP  - 2
DP  - 2005 May 3
TI  - Effects of prenatal alcohol exposure on brain-derived neurotrophic factor and its 
      receptor tyrosine kinase B in offspring.
PG  - 125-32
AB  - Prenatal alcohol exposure produces many developmental defects in the central 
      nervous system. The underlying molecular mechanism, however, has not been fully 
      understood. The present study was undertaken to examine the effects of prenatal 
      alcohol exposure on brain-derived neurotrophic factor (BDNF) and its receptor 
      tyrosine kinase B (TrkB) in offspring. The pregnant Sprague-Dawley rats received 
      1 or 3 g/kg of alcohol or an isocaloric solution by intragastric intubation once 
      a day from gestational day (GD) 5 to GD 20. On postnatal day 7-8, pups were 
      killed and the hippocampus, striatum, cortex, and cerebellum dissected out. 
      Levels of BDNF mRNA and proteins, total TrkB proteins and receptor 
      phosphorylation were measured. The results showed that prenatal alcohol exposure 
      at the dose of 1 g/kg/day did not significantly affect BDNF protein levels in any 
      region examined. However, administration of alcohol at the dose of 3 g/kg/day 
      markedly reduced levels of BDNF protein and mRNA in the cortex and hippocampus of 
      offspring. Western blotting showed that prenatal alcohol exposure at the dose of 
      3 g/kg/day also inhibited TrkB phosphorylation in the hippocampus although no 
      changes in total TrkB protein levels were observed in any region examined. Our 
      data suggest that prenatal alcohol exposure alters both presynaptic and 
      postsynaptic BDNF function in certain brain areas of offspring. These alterations 
      in BDNF function may contribute to the development of alcohol-related birth 
      defects.
FAU - Feng, Mei-Jiang
AU  - Feng MJ
AD  - Department of Biomedical and Therapeutic Sciences, University of Illinois College 
      of Medicine at Peoria, Peoria, IL 61656, USA.
FAU - Yan, Shu-E
AU  - Yan SE
FAU - Yan, Qing-Shan
AU  - Yan QS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 3K9958V90M (Ethanol)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Ethanol/*administration & dosage
MH  - Female
MH  - Male
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/genetics/*metabolism
EDAT- 2005/04/28 09:00
MHDA- 2005/07/16 09:00
CRDT- 2005/04/28 09:00
PHST- 2004/12/13 00:00 [received]
PHST- 2005/02/03 00:00 [revised]
PHST- 2005/02/04 00:00 [accepted]
PHST- 2005/04/28 09:00 [pubmed]
PHST- 2005/07/16 09:00 [medline]
PHST- 2005/04/28 09:00 [entrez]
AID - S0006-8993(05)00244-1 [pii]
AID - 10.1016/j.brainres.2005.02.017 [doi]
PST - ppublish
SO  - Brain Res. 2005 May 3;1042(2):125-32. doi: 10.1016/j.brainres.2005.02.017.