PMID- 15854640
OWN - NLM
STAT- MEDLINE
DCOM- 20060509
LR  - 20131121
IS  - 0010-7824 (Print)
IS  - 0010-7824 (Linking)
VI  - 71
IP  - 5
DP  - 2005 May
TI  - Cyproterone acetate affects protamine gene expression in the testis of adult male 
      rat.
PG  - 379-91
AB  - The temporal effects of oral administration of cyproterone acetate (CPA), a 
      progestational androgen receptor blocker, were studied on the fertility of adult 
      male rat sires, at a dose of 20 mg kg-1 day-1 after 15 days of gavage. The 
      treatment reduced the fertility and weights of accessory sex glands, without 
      altering the serum levels of luteinizing hormone, follicle-stimulating hormone 
      (FSH) and testosterone (T). Sperm counts were significantly reduced after 
      treatment. Several changes were evident in caput epididymal sperm chromatin in 
      treated rats. The in vitro decondensation rates of sperm chromatin and total 
      fluorescent acridine orange (AO) dye uptake were enhanced. The fluorescent AO dye 
      uptake by the double- and single-stranded sperm chromatin increased. The uptake 
      of thiol-specific monobromobimane fluorescent dye by sperm chromatin was 
      significantly reduced. Sperm of treated rats exhibited hypoprotamination. 
      Protamine levels in the testis were significantly reduced after treatment. 
      Androgen-binding protein (ABP) expression was significantly reduced in testis 
      after treatment. A slight but significant increase was observed in cyclic AMP 
      immunoexpression in testis after treatment. The expression and levels of 
      transition proteins 1 (TP1) and 2 (TP2) as well as cyclic AMP response element 
      modulator protein-tau were maintained at control levels in the testis of treated 
      rats. The present study reports that androgen receptor occupation by CPA 
      preferentially reduces the levels of spermatidal protamine in testis and 
      spermatozoa involved in nuclear chromatin condensation. It is inferred that ABP 
      could be mediating the effects of T in modulating the sequential expression of 
      TPs and protamines during nuclear chromatin condensation. It is likely that 
      indirect effects of T involve its aromatization in spermatids.
FAU - Aleem, Mukhtar
AU  - Aleem M
AD  - Department of Neuroendocrinology, National Institute for Research in Reproductive 
      Health (ICMR), Mumbai 400 012, India.
FAU - Padwal, Varsha
AU  - Padwal V
FAU - Choudhari, Jyoti
AU  - Choudhari J
FAU - Balasinor, Nafisa
AU  - Balasinor N
FAU - Parte, Priyanka
AU  - Parte P
FAU - Gill-Sharma, Manjeet
AU  - Gill-Sharma M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Contraception
JT  - Contraception
JID - 0234361
RN  - 0 (Androgen Antagonists)
RN  - 0 (Contraceptive Agents, Male)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Protamines)
RN  - 135844-64-3 (Cyclic AMP Response Element Modulator)
RN  - 3XMK78S47O (Testosterone)
RN  - 4KM2BN5JHF (Cyproterone Acetate)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Androgen Antagonists/*pharmacology
MH  - Animals
MH  - Contraceptive Agents, Male/*pharmacology
MH  - Cyclic AMP/metabolism
MH  - Cyclic AMP Response Element Modulator
MH  - Cyproterone Acetate/*pharmacology
MH  - DNA-Binding Proteins/metabolism
MH  - Gene Expression
MH  - Male
MH  - Protamines/*genetics/metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sex Chromatin/*metabolism
MH  - Sperm Count
MH  - Spermatogenesis/physiology
MH  - Spermatozoa/drug effects
MH  - Testis/*drug effects/metabolism
MH  - Testosterone/physiology
EDAT- 2005/04/28 09:00
MHDA- 2006/05/10 09:00
CRDT- 2005/04/28 09:00
PHST- 2004/08/13 00:00 [received]
PHST- 2004/11/05 00:00 [accepted]
PHST- 2005/04/28 09:00 [pubmed]
PHST- 2006/05/10 09:00 [medline]
PHST- 2005/04/28 09:00 [entrez]
AID - S0010-7824(04)00306-3 [pii]
AID - 10.1016/j.contraception.2004.11.003 [doi]
PST - ppublish
SO  - Contraception. 2005 May;71(5):379-91. doi: 10.1016/j.contraception.2004.11.003.