PMID- 15856159
OWN - NLM
STAT- MEDLINE
DCOM- 20050921
LR  - 20181113
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 111
IP  - 1
DP  - 2005 Jun
TI  - The L locus, one of complementary genes required for anthocyanin production in 
      onions (Allium cepa), encodes anthocyanidin synthase.
PG  - 120-7
AB  - Bulb color in onions (Allium cepa) is an important trait, but its complex, 
      unclear mechanism of inheritance has been a limiting factor in onion cultivar 
      improvement. The identity of the L locus, which is involved in the color 
      difference between Brazilian yellow and red onions, is revealed in this study. A 
      cross was made between a US-type yellow breeding line and a Brazilian yellow 
      cultivar. The segregation ratio of nine red to seven yellow onions in the F(2) 
      population supports the involvement of two complementary genes in anthocyanin 
      production in the F(1) hybrids. The high-performance liquid chromatography (HPLC) 
      and reverse-transcriptase (RT)-PCR analysis of the Brazilian yellow onions 
      indicated that the genes are involved late in the anthocyanin synthesis pathway. 
      The genomic sequence of the anthocyanidin synthase (ANS) gene in Brazilian yellow 
      onions showed a point mutation, which results in an amino acid change of a 
      glycine to an arginine at residue 229. Because this residue is located adjacent 
      to a highly conserved iron-binding active site, this mutation is likely 
      responsible for the inactivation of the ANS gene in Brazilian yellow onions. 
      Following the isolation of the promoter sequence of the mutant allele, a 
      PCR-based marker for allelic selection of the ANS gene was designed. This assay 
      is based on an insertion (larger than 3 kb) mutation. The marker perfectly 
      co-segregated with the color phenotypes in the F(2) populations, thereby 
      indicating that the L locus encodes ANS.
FAU - Kim, Sunggil
AU  - Kim S
AD  - Department of Horticultural Sciences, Vegetable & Fruit Improvement Center, Texas 
      A&M University, College Station, TX 77845, USA.
FAU - Jones, Rick
AU  - Jones R
FAU - Yoo, Kil-Sun
AU  - Yoo KS
FAU - Pike, Leonard M
AU  - Pike LM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20050426
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (Anthocyanins)
RN  - 0 (DNA Primers)
RN  - EC 1.13.- (Oxygenases)
RN  - EC 1.14.99.- (anthocyanidin synthase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anthocyanins/*biosynthesis/genetics
MH  - Base Sequence
MH  - Chromatography, High Pressure Liquid
MH  - Crosses, Genetic
MH  - DNA Primers
MH  - Gene Components
MH  - Molecular Sequence Data
MH  - Mutation, Missense/genetics
MH  - Onions/enzymology/*genetics
MH  - Oxygenases/*genetics
MH  - Pigmentation/*genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2005/04/28 09:00
MHDA- 2005/09/22 09:00
CRDT- 2005/04/28 09:00
PHST- 2004/11/29 00:00 [received]
PHST- 2005/03/08 00:00 [accepted]
PHST- 2005/04/28 09:00 [pubmed]
PHST- 2005/09/22 09:00 [medline]
PHST- 2005/04/28 09:00 [entrez]
AID - 10.1007/s00122-005-2000-1 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2005 Jun;111(1):120-7. doi: 10.1007/s00122-005-2000-1. Epub 
      2005 Apr 26.