PMID- 15856293
OWN - NLM
STAT- MEDLINE
DCOM- 20050927
LR  - 20181113
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 446
IP  - 6
DP  - 2005 Jun
TI  - Vascular endothelial growth factor in salivary pleomorphic adenomas: one of the 
      reasons for their poorly vascularized stroma.
PG  - 653-62
AB  - To better understand the poorly vascularized background of the stroma of 
      pleomorphic adenomas, we attempted to determine the expression of molecules 
      related to blood vessels and hypoxic conditions in pleomorphic adenoma. Surgical 
      specimens and tumor cells in primary culture of salivary pleomorphic adenomas 
      were used for immunohistochemistry for CD31, vascular endothelial growth factor 
      (VEGF) and its receptors Flk-1 and Flt-1, as well as for hypoxia markers, such as 
      hypoxia-inducible factor-1alpha (HIF-1alpha) and lactate dehydrogenase-1 (LDH). 
      At the same time, alternative splicing modes of the VEGF gene and expression 
      levels of the HIF-1alpha gene were analyzed in surgical specimens by means of 
      reverse-transcription polymerase chain reaction (RT-PCR) and direct sequencing of 
      the PCR products. In addition to co-immunolocalization with CD31+ vascular 
      endothelial cells, VEGF and its receptors were demonstrated in normal duct 
      epithelial and myoepithelial cells as well as in tumor cells in ductal structures 
      and in myxochondroid stromata. Immunolocalizations for HIF-1alpha and LDH were 
      confirmed in the VEGF-positive area. Immunofluorescence signals for VEGF and 
      others were confirmed in pleomorphic adenoma cells in culture. RT-PCR results 
      showed that there were at least four splicing modes of the VEGF gene, among which 
      VEGF(121) was most enhanced, and higher HIF-1alpha levels in pleomorphic 
      adenomas. The results suggest that pleomorphic adenoma cells produce VEGF in 
      several functional forms for their own proliferation or differentiation, and that 
      the VEGF expression is controlled by hypoxic circumstances of poorly vascularized 
      pleomorphic adenomas.
FAU - Swelam, Wael
AU  - Swelam W
AD  - Department of Tissue Regeneration and Reconstruction, Division of Oral Pathology, 
      Niigata University Graduate School of Medical and Dental Sciences, 2-5274 
      Gakkocho-dori, Niigata 951-8514, Japan.
FAU - Ida-Yonemochi, Hiroko
AU  - Ida-Yonemochi H
FAU - Maruyama, Satoshi
AU  - Maruyama S
FAU - Ohshiro, Kazufumi
AU  - Ohshiro K
FAU - Cheng, Jun
AU  - Cheng J
FAU - Saku, Takashi
AU  - Saku T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050423
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.10.1 (FGFR1 protein, human)
RN  - EC 2.7.10.1 (FLT1 protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
SB  - IM
MH  - Adenoma, Pleomorphic/*blood supply/metabolism
MH  - Alternative Splicing
MH  - Biomarkers, Tumor/analysis
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Hypoxia/metabolism/*physiopathology
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH  - Proteins/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Receptor, Fibroblast Growth Factor, Type 1
MH  - Receptors, Fibroblast Growth Factor/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Salivary Gland Neoplasms/*blood supply/metabolism
MH  - Transcription Factors/metabolism
MH  - Vascular Endothelial Growth Factor A/*genetics/*metabolism
MH  - Vascular Endothelial Growth Factor Receptor-1
EDAT- 2005/04/28 09:00
MHDA- 2005/09/28 09:00
CRDT- 2005/04/28 09:00
PHST- 2004/11/11 00:00 [received]
PHST- 2005/01/24 00:00 [accepted]
PHST- 2005/04/28 09:00 [pubmed]
PHST- 2005/09/28 09:00 [medline]
PHST- 2005/04/28 09:00 [entrez]
AID - 10.1007/s00428-005-1219-1 [doi]
PST - ppublish
SO  - Virchows Arch. 2005 Jun;446(6):653-62. doi: 10.1007/s00428-005-1219-1. Epub 2005 
      Apr 23.