PMID- 15858001
OWN - NLM
STAT- MEDLINE
DCOM- 20050620
LR  - 20240510
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 79
IP  - 10
DP  - 2005 May
TI  - The locus encompassing the latency-associated transcript of herpes simplex virus 
      type 1 interferes with and delays interferon expression in productively infected 
      neuroblastoma cells and trigeminal Ganglia of acutely infected mice.
PG  - 6162-71
AB  - The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) is 
      the only abundant viral transcript expressed in latently infected neurons. LAT 
      inhibits apoptosis, suggesting that it regulates latency by promoting the 
      survival of infected neurons. The LAT locus also contains a newly described gene 
      (AL), which is antisense to LAT and partially overlaps LAT encoding sequences. 
      When human (SK-N-SH) or mouse (neuro-2A) neuroblastoma cells were infected with a 
      virus that does not express LAT or AL gene products (dLAT2903), beta interferon 
      (IFN-beta) and IFN-alpha RNA expression was detected earlier relative to the same 
      cells infected with HSV-1 strains that express LAT and AL. Infection of neuro-2A 
      cells with dLAT2903 also led to higher levels of IFN-beta promoter activity than 
      in cells infected with wild-type (wt) HSV-1. In contrast, IFN RNA expression was 
      the same when human lung fibroblasts were infected with dLAT2903 or wt HSV-1. 
      When BALB/c mice were infected with dLAT2903, IFN-alpha and IFN-beta RNA 
      expression was readily detected in trigeminal ganglia (TG) 4 days after 
      infection. These transcripts were not detected in TG of mice infected with wt 
      HSV-1 or dLAT2903R (marker-rescued dLAT2903) until 6 days postinfection. When TG 
      single-cell suspensions from infected BALB/c mice were prepared and incubated in 
      vitro with wt HSV-1 as a source of antigen, TG cultures prepared from mice 
      infected with dLAT2903 produced and secreted higher levels of IFN protein than wt 
      HSV-1 or dLAT2903R. Collectively, these studies suggest that the LAT locus 
      interferes with and delays IFN expression.
FAU - Peng, Weiping
AU  - Peng W
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      University of Nebraska, Lincoln, Fair Street at East Campus Loop, Lincoln, NE 
      68583-0905, USA.
FAU - Henderson, Gail
AU  - Henderson G
FAU - Inman, Melissa
AU  - Inman M
FAU - BenMohamed, Lbachir
AU  - BenMohamed L
FAU - Perng, Guey-Chuen
AU  - Perng GC
FAU - Wechsler, Steven L
AU  - Wechsler SL
FAU - Jones, Clinton
AU  - Jones C
LA  - eng
GR  - 1P20RR15635/RR/NCRR NIH HHS/United States
GR  - R01 EY012823/EY/NEI NIH HHS/United States
GR  - R01 EY013191/EY/NEI NIH HHS/United States
GR  - EY13701/EY/NEI NIH HHS/United States
GR  - EY13191/EY/NEI NIH HHS/United States
GR  - EY12823/EY/NEI NIH HHS/United States
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Interferon-alpha)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Viral Proteins)
RN  - 0 (latency associated transcript, herpes simplex virus-1)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Herpes Simplex/*immunology
MH  - Herpesvirus 1, Human/genetics/*immunology
MH  - Humans
MH  - Interferon-alpha/*biosynthesis/genetics
MH  - Interferon-beta/*biosynthesis/genetics
MH  - Mice
MH  - MicroRNAs
MH  - Neuroblastoma/immunology/virology
MH  - RNA, Messenger/analysis
MH  - Time Factors
MH  - Trigeminal Ganglion/immunology/*virology
MH  - Viral Proteins/*immunology
PMC - PMC1091686
EDAT- 2005/04/29 09:00
MHDA- 2005/06/21 09:00
PMCR- 2005/05/01
CRDT- 2005/04/29 09:00
PHST- 2005/04/29 09:00 [pubmed]
PHST- 2005/06/21 09:00 [medline]
PHST- 2005/04/29 09:00 [entrez]
PHST- 2005/05/01 00:00 [pmc-release]
AID - 79/10/6162 [pii]
AID - 1972-04 [pii]
AID - 10.1128/JVI.79.10.6162-6171.2005 [doi]
PST - ppublish
SO  - J Virol. 2005 May;79(10):6162-71. doi: 10.1128/JVI.79.10.6162-6171.2005.