PMID- 15858958
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20190410
IS  - 0803-5253 (Print)
IS  - 0803-5253 (Linking)
VI  - 94
IP  - 1
DP  - 2005 Jan
TI  - Determination of the six major human herpesviruses in cerebrospinal fluid and 
      blood specimens of children.
PG  - 38-43
AB  - AIM: To detect and differentiate six major human herpesviruses in the 
      cerebrospinal fluid (CSF) and blood of children by polymerase chain reaction 
      restriction fragment length polymorphism (PCR-RFLP). METHODS: We synthesized two 
      pairs of primers in the well-conserved regions of the DNA polymerase gene in 
      human herpesviruses. One pair was designed to amplify cytomegalovirus (CMV), 
      Epstein-Barr virus (EBV), herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), 
      and the other pair to amplify varicella-zoster virus (VZV) and human herpesvirus 
      6 (HHV-6) by PCR. Virus species identification was achieved by restriction enzyme 
      digestion with BamHI and BstUI. Ninety-eight CSF and 75 blood specimens were 
      analysed by this technique. At the same time, all blood specimens were also 
      examined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Thirteen (13.3%) 
      of 98 CSF specimens and 26 (34.7%) of 75 blood specimens were positive for 
      herpesvirus DNA in this PCR assay. Only 10 (13.3%) of the blood specimens were 
      positive in ELISA for virus-IgM antibody. Sensitivity, specificity, positive 
      predictive value (PPV) and negative predictive value (NPV) of PCR in detecting 
      herpesvirus infections compared with ELISA were 100% (10/10), 75.4% (49/65), 
      38.5% (10/26) and 100% (49/49), respectively. These results indicate that the 
      positive rate of PCR was significantly higher than that of ELISA (p < 0.05). The 
      herpesvirus type of these positive specimens was rapidly detected using 
      restriction enzyme digestion with BamHI and BstUI. CONCLUSIONS: PCR-RFLP is a 
      specific, sensitive and accurate technique for the identification of herpesvirus 
      infections in the CSF and blood of children.
FAU - Dong, G
AU  - Dong G
AD  - Department of Infectious Medicine, Children's Hospital of Zhejiang University, 
      School of Medicine, Hangzhou, China. dongguanp@yahoo.com.cn
FAU - Shang, S
AU  - Shang S
FAU - Liang, L
AU  - Liang L
FAU - Yu, X
AU  - Yu X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Norway
TA  - Acta Paediatr
JT  - Acta paediatrica (Oslo, Norway : 1992)
JID - 9205968
RN  - 0 (DNA Primers)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
MH  - Betaherpesvirinae/genetics/*isolation & purification
MH  - Central Nervous System Viral Diseases/*blood/*cerebrospinal fluid
MH  - DNA Primers
MH  - DNA-Directed DNA Polymerase/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Herpesvirus 3, Human/genetics/*isolation & purification
MH  - Herpesvirus 4, Human/genetics/*isolation & purification
MH  - Humans
MH  - Infant
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Simplexvirus/genetics/*isolation & purification
EDAT- 2005/04/30 09:00
MHDA- 2005/06/09 09:00
CRDT- 2005/04/30 09:00
PHST- 2005/04/30 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/04/30 09:00 [entrez]
AID - 10.1111/j.1651-2227.2005.tb01785.x [doi]
PST - ppublish
SO  - Acta Paediatr. 2005 Jan;94(1):38-43. doi: 10.1111/j.1651-2227.2005.tb01785.x.