PMID- 15860669
OWN - NLM
STAT- MEDLINE
DCOM- 20051128
LR  - 20221207
IS  - 0006-4971 (Print)
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 106
IP  - 7
DP  - 2005 Oct 1
TI  - Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human 
      dendritic cells.
PG  - 2366-74
AB  - Lymphopenia and increasing viral load in the first 10 days of severe acute 
      respiratory syndrome (SARS) suggested immune evasion by SARS-coronavirus (CoV). 
      In this study, we focused on dendritic cells (DCs) which play important roles in 
      linking the innate and adaptive immunity. SARS-CoV was shown to infect both 
      immature and mature human monocyte-derived DCs by electron microscopy and 
      immunofluorescence. The detection of negative strands of SARS-CoV RNA in DCs 
      suggested viral replication. However, no increase in viral RNA was observed. 
      Using cytopathic assays, no increase in virus titer was detected in infected DCs 
      and cell-culture supernatant, confirming that virus replication was incomplete. 
      No induction of apoptosis or maturation was detected in SARS-CoV-infected DCs. 
      The SARS-CoV-infected DCs showed low expression of antiviral cytokines 
      (interferon alpha [IFN-alpha], IFN-beta, IFN-gamma, and interleukin 12p40 
      [IL-12p40]), moderate up-regulation of proinflammatory cytokines (tumor necrosis 
      factor alpha [TNF-alpha] and IL-6) but significant up-regulation of inflammatory 
      chemokines (macrophage inflammatory protein 1alpha [MIP-1alpha], regulated on 
      activation normal T cell expressed and secreted [RANTES]), interferon-inducible 
      protein of 10 kDa [IP-10], and monocyte chemoattractant protein 1 [MCP-1]). The 
      lack of antiviral cytokine response against a background of intense chemokine 
      up-regulation could represent a mechanism of immune evasion by SARS-CoV.
FAU - Law, Helen K W
AU  - Law HK
AD  - Department of Paediatrics and Adolescent Medicine, Hong Kong Jockey Club Clinical 
      Research Centre, Faculty of Medicine, The University of Hong Kong, Queen Mary 
      Hospital, Pokfulam, Hong Kong, China.
FAU - Cheung, Chung Yan
AU  - Cheung CY
FAU - Ng, Hoi Yee
AU  - Ng HY
FAU - Sia, Sin Fun
AU  - Sia SF
FAU - Chan, Yuk On
AU  - Chan YO
FAU - Luk, Winsie
AU  - Luk W
FAU - Nicholls, John M
AU  - Nicholls JM
FAU - Peiris, J S Malik
AU  - Peiris JS
FAU - Lau, Yu Lung
AU  - Lau YL
LA  - eng
GR  - N01AI95357/AI/NIAID NIH HHS/United States
GR  - AI95357/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050428
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Interleukin-6)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Viral)
RN  - 187348-17-0 (Interleukin-12)
RN  - 63231-63-0 (RNA)
RN  - 77238-31-4 (Interferon-beta)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis
MH  - Caspase 3
MH  - Caspases/biosynthesis
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/biosynthesis
MH  - Chemokines/*biosynthesis
MH  - Cytokines/metabolism
MH  - DNA Primers/chemistry
MH  - Dendritic Cells/*cytology
MH  - Enzyme Activation
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Inflammation
MH  - Interferon-alpha/biosynthesis
MH  - Interferon-beta/biosynthesis
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-12/biosynthesis/metabolism
MH  - Interleukin-12 Subunit p40
MH  - Interleukin-6/biosynthesis
MH  - Leukocytes, Mononuclear/cytology
MH  - Macrophage Inflammatory Proteins/biosynthesis
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Monocytes/*cytology
MH  - Polymerase Chain Reaction
MH  - Protein Subunits/biosynthesis
MH  - RNA/metabolism
MH  - RNA, Viral/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Severe acute respiratory syndrome-related coronavirus/genetics/*metabolism
MH  - Time Factors
MH  - *Up-Regulation
PMC - PMC1895271
EDAT- 2005/04/30 09:00
MHDA- 2005/12/13 09:00
PMCR- 2020/12/14
CRDT- 2005/04/30 09:00
PHST- 2005/04/30 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/04/30 09:00 [entrez]
PHST- 2020/12/14 00:00 [pmc-release]
AID - S0006-4971(20)67229-9 [pii]
AID - 10.1182/blood-2004-10-4166 [doi]
PST - ppublish
SO  - Blood. 2005 Oct 1;106(7):2366-74. doi: 10.1182/blood-2004-10-4166. Epub 2005 Apr 
      28.