PMID- 15860795
OWN - NLM
STAT- MEDLINE
DCOM- 20050913
LR  - 20220316
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 33
IP  - 2
DP  - 2005 Aug
TI  - Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is 
      mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and 
      nuclear factor-kappaB.
PG  - 186-94
AB  - Interleukin (IL)-25, a novel Th2 cytokine, is capable of amplifying allergic 
      inflammation. We investigated the modulation of nuclear factor (NF)-kappaB and 
      mitogen-activated protein kinases (MAPK) pathways in IL-25-activated eosinophils, 
      the principal effector cells of allergic inflammation, for the in vitro release 
      of chemokines including monocyte chemoattractant protein-1 (MCP-1), IL-8, and 
      macrophage inflammatory protein (MIP)-1alpha, and inflammatory cytokine IL-6. 
      Gene expression of chemokines and IL-6 was evaluated by RT-PCR, and 
      concentrations of chemokines and cytokine were measured by cytokine protein 
      array, cytometric bead array, and enzyme-linked immunosorbent assay. NF-kappaB, 
      c-Jun amino-terminal kinase (JNK), and p38 MAPK activities in eosinophils were 
      assessed by electrophoretic mobility shift assay and Western blot. IL-25 was 
      found to upregulate the gene expression of chemokines MCP-1, MIP-1alpha, and 
      IL-8, and cytokine IL-6, in eosinophils, and to significantly increase the 
      release of the above chemokines and IL-6 from eosinophils. IL-25 could also 
      activate the JNK, p38 MAPK, and NF-kappaB activities of eosinophils, while 
      inhibitor of IkappaB-alpha phosphorylation (BAY11-7082), JNK (SP600125), and p38 
      MAPK (SB203580) could suppress the release of IL-8, MIP-1alpha, MCP-1, and IL-6. 
      Together, the above results showed that the induction of MCP-1, MIP-1alpha, IL-8, 
      and IL-6 in IL-25-activated eosinophils are regulated by JNK, p38 MAPK, and 
      NF-kappaB pathways.
FAU - Wong, Chun K
AU  - Wong CK
AD  - Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of 
      Wales Hospital, Shatin, Hong Kong.
FAU - Cheung, Phyllis F Y
AU  - Cheung PF
FAU - Ip, Wai K
AU  - Ip WK
FAU - Lam, Christopher W K
AU  - Lam CW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050428
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (3-(4-methylphenylsulfonyl)-2-propenenitrile)
RN  - 0 (Anthracenes)
RN  - 0 (Chemokines)
RN  - 0 (DNA, Complementary)
RN  - 0 (IL25 protein, human)
RN  - 0 (Imidazoles)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukins)
RN  - 0 (NF-kappa B)
RN  - 0 (Nitriles)
RN  - 0 (Pyridines)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sulfones)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 1TW30Y2766 (pyrazolanthrone)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Anthracenes/pharmacology
MH  - Base Sequence
MH  - Chemokines/*biosynthesis/genetics
MH  - DNA, Complementary/genetics
MH  - Eosinophils/*drug effects/*immunology/metabolism
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - In Vitro Techniques
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-17
MH  - Interleukin-6/*biosynthesis/genetics
MH  - Interleukins/*pharmacology
MH  - JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - NF-kappa B/antagonists & inhibitors/metabolism
MH  - Nitriles/pharmacology
MH  - Pyridines/pharmacology
MH  - Receptors, Interleukin/metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Sulfones/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
EDAT- 2005/04/30 09:00
MHDA- 2005/09/15 09:00
CRDT- 2005/04/30 09:00
PHST- 2005/04/30 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/04/30 09:00 [entrez]
AID - 2005-0034OC [pii]
AID - 10.1165/rcmb.2005-0034OC [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2005 Aug;33(2):186-94. doi: 10.1165/rcmb.2005-0034OC. 
      Epub 2005 Apr 28.