PMID- 15861816
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20191210
IS  - 1438-4221 (Print)
IS  - 1438-4221 (Linking)
VI  - 295
IP  - 1
DP  - 2005 Apr
TI  - Rapid detection and identification of pathogens in blood cultures by fluorescence 
      in situ hybridization and flow cytometry.
PG  - 47-55
AB  - Septicemia is one of the leading causes of death in hospitalized patients. The 
      timely detection and identification of microorganisms from the patient's blood 
      has great diagnostic, prognostic and economic significance. Fluorescence in situ 
      hybridization (FISH) with rRNA-targeted oligonucleotide probes has been proven to 
      be a fast method for the identification of human pathogenic bacteria and yeasts. 
      Data presented herein reveal that the combination of FISH and flow cytometry 
      (FC-FISH) is a rapid and reliable technique for identification of pathogens 
      (Gram-negative rods, Candida spp.) directly from blood cultures without further 
      cultivation and biotyping. Moreover, detection of growing pathogens (e.g., 
      Stenotrophomonas maltophilia) in blood cultures is achieved more rapidly by 
      FC-FISH compared to standard detection methods. Therefore, FC-FISH allows rapid 
      detection and identification of pathogens in blood cultures.
FAU - Kempf, Volkhard A J
AU  - Kempf VA
AD  - Institut fur Medizinische Mikrobiologie und Hygiene, Eberhard-Karls-Universitat 
      Tubingen, Elfriede-Aulhorn Str. 6, D-72076 Tubingen, Germany. 
      volkhard.kempf@med.uni-tuebingen.de
FAU - Mandle, Tanja
AU  - Mandle T
FAU - Schumacher, Ulrike
AU  - Schumacher U
FAU - Schafer, Andrea
AU  - Schafer A
FAU - Autenrieth, Ingo B
AU  - Autenrieth IB
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
SB  - IM
MH  - Bacteria/classification/*isolation & purification
MH  - Bacteriological Techniques/*methods
MH  - Blood/*microbiology
MH  - Candida/classification/isolation & purification
MH  - Clinical Protocols
MH  - *Flow Cytometry
MH  - Fungi/classification/*isolation & purification
MH  - Germany
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pilot Projects
MH  - Sepsis/microbiology
MH  - Stenotrophomonas maltophilia/classification/isolation & purification
MH  - Time Factors
EDAT- 2005/05/03 09:00
MHDA- 2005/06/09 09:00
CRDT- 2005/05/03 09:00
PHST- 2005/05/03 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/05/03 09:00 [entrez]
AID - S1438-4221(04)00155-9 [pii]
AID - 10.1016/j.ijmm.2004.12.006 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2005 Apr;295(1):47-55. doi: 10.1016/j.ijmm.2004.12.006.