PMID- 15863253
OWN - NLM
STAT- MEDLINE
DCOM- 20050620
LR  - 20151119
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 211
IP  - 1-2
DP  - 2005 Jul 1
TI  - Use of the local lymph node assay in assessment of immune function.
PG  - 107-14
AB  - The murine local lymph node assay (LLNA) was originally developed as a predictive 
      test method for the identification of chemicals with sensitizing potential. In 
      this study we demonstrated that an adapted LLNA can also be used as an immune 
      function assay by studying the effects of orally administered immunomodulating 
      compounds on the T-cell-dependent immune response induced by the contact 
      sensitizer 2,4-dinitrochlorobenzene (DNCB). C57Bl/6 mice were treated with the 
      immunotoxic compounds cyclosporin A (CsA), bis(tri-n-butyltin)oxide (TBTO) or 
      benzo[a]pyrene, (B[a]P). Subsequently, cell proliferation and interferon-gamma 
      (IFN-gamma) and interleukin (IL)-4 release were determined in the auricular lymph 
      nodes (LNs) after DNCB application on both ears. Immunosuppression induced by 
      CsA, TBTO and B[a]P was clearly detectable in this application of the LLNA. 
      Cytokine release measurements proved valuable to confirm the results of the cell 
      proliferation assay and to obtain an indication of the effect on Th1/Th2 balance. 
      We believe to have demonstrated the applicability of an adapted LLNA as an immune 
      function assay in the mouse.
FAU - van den Berg, Femke A
AU  - van den Berg FA
AD  - National Institute of Public Health and the Environment (RIVM), Department of 
      Toxicology, Pathology and Genetics, Postbus 1, 3720 BA Bilthoven, The 
      Netherlands. Femke.van.den.Berg@rivm.nl
FAU - Baken, Kirsten A
AU  - Baken KA
FAU - Vermeulen, Jolanda P
AU  - Vermeulen JP
FAU - Gremmer, Eric R
AU  - Gremmer ER
FAU - van Steeg, Harry
AU  - van Steeg H
FAU - van Loveren, Henk
AU  - van Loveren H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050407
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Cytokines)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Trialkyltin Compounds)
RN  - 3353Q84MKM (bis(tri-n-butyltin)oxide)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Benzo(a)pyrene/pharmacology
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cyclosporine/pharmacology
MH  - Cytokines/metabolism
MH  - Female
MH  - Immunosuppressive Agents/*pharmacology
MH  - Interferon-gamma/pharmacology
MH  - *Local Lymph Node Assay
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred CBA
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
MH  - Thymidine/metabolism
MH  - Trialkyltin Compounds/pharmacology
EDAT- 2005/05/03 09:00
MHDA- 2005/06/21 09:00
CRDT- 2005/05/03 09:00
PHST- 2005/01/27 00:00 [received]
PHST- 2005/03/03 00:00 [revised]
PHST- 2005/03/03 00:00 [accepted]
PHST- 2005/05/03 09:00 [pubmed]
PHST- 2005/06/21 09:00 [medline]
PHST- 2005/05/03 09:00 [entrez]
AID - S0300-483X(05)00148-4 [pii]
AID - 10.1016/j.tox.2005.03.003 [doi]
PST - ppublish
SO  - Toxicology. 2005 Jul 1;211(1-2):107-14. doi: 10.1016/j.tox.2005.03.003. Epub 2005 
      Apr 7.