PMID- 15863260
OWN - NLM
STAT- MEDLINE
DCOM- 20050620
LR  - 20191210
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 211
IP  - 1-2
DP  - 2005 Jul 1
TI  - Quantitative comparison of the results obtained by the multiple-dose guinea pig 
      maximization test and the non-radioactive murine local lymph-node assay for 
      various biocides.
PG  - 165-75
AB  - We compared the results of the multiple-dose guinea pig maximization test (GPMT) 
      and the non-radioactive murine local lymph-node assay (LLNA) for various 
      biocides. Thirteen out of 17 positive biocides in the GPMT gave positive results 
      in the LLNA. In the GPMT, the minimum first induction doses ranged over four 
      orders (0.00005-0.5%), while elicitation-threshold doses, which were evaluated 
      using an optimally sensitized group of animals in the multiple-dose studies, 
      ranged over five orders (0.00006-2.8%). In the LLNA, minimum induction doses 
      ranged over more than three orders (0.01-30%). With respect to 13 biocides that 
      were positive in both the GPMT and the LLNA, results were quantitatively 
      compared. When compared after conversion to corresponding area doses (microg/cm), 
      the minimum doses required to elicit skin reaction in guinea pigs were always 
      lower than that for induction in mice with all biocides. Correlation between 
      minimum induction doses from the GPMT and the LLNA seemed poor (r=0.57), while 
      that between minimum induction doses in the LLNA and elicitation-threshold doses 
      in the GPMT was relatively good (r=0.73). The results suggest the possibility to 
      estimate human elicitation-threshold doses, which are definitely lacking in the 
      process of risk assessment for skin-sensitizers, from the data of the LLNA.
FAU - Yamano, Tetsuo
AU  - Yamano T
AD  - Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, 
      Tennoji-ku, Osaka 543-0026, Japan. tetsuo.yamano@iphes.city.osaka.jp
FAU - Shimizu, Mitsuru
AU  - Shimizu M
FAU - Noda, Tsutomu
AU  - Noda T
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20050401
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Antimetabolites)
RN  - 0 (Xenobiotics)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Antimetabolites
MH  - Bromodeoxyuridine
MH  - Chemical Phenomena
MH  - Chemistry, Physical
MH  - Dermatitis, Allergic Contact/*diagnosis
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Guinea Pigs
MH  - Injections, Intradermal
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Skin/*pathology
MH  - Xenobiotics/administration & dosage/chemistry/*toxicity
EDAT- 2005/05/03 09:00
MHDA- 2005/06/21 09:00
CRDT- 2005/05/03 09:00
PHST- 2004/12/15 00:00 [received]
PHST- 2005/02/19 00:00 [accepted]
PHST- 2005/05/03 09:00 [pubmed]
PHST- 2005/06/21 09:00 [medline]
PHST- 2005/05/03 09:00 [entrez]
AID - S0300-483X(05)00144-7 [pii]
AID - 10.1016/j.tox.2005.02.014 [doi]
PST - ppublish
SO  - Toxicology. 2005 Jul 1;211(1-2):165-75. doi: 10.1016/j.tox.2005.02.014. Epub 2005 
      Apr 1.