PMID- 15865295
OWN - NLM
STAT- MEDLINE
DCOM- 20050616
LR  - 20061115
IS  - 0016-6758 (Print)
IS  - 0016-6758 (Linking)
VI  - 41
IP  - 3
DP  - 2005 Mar
TI  - [Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells 
      of humans exposed to adverse environmental factors].
PG  - 391-5
AB  - Fluorescence in situ hybridization (FISH) was used to compare aneuploidy rates in 
      four autosomes and two sex chromosomes in interphase nuclei of noncultivated 
      (quiescent) and cultivated (induced to divide with phytohemagglutinin (PHA)) 
      leukocytes in people engaged in nuclear-chemical industry and in a control group 
      of people not exposed to mutagenic factors occupationally or at home. The overall 
      rates of numerical chromosome aberrations for all of the six chromosomes studied 
      showed little difference, although a higher rate of loss of the X- and 
      Y-chromosomes was observed in the exposed group. In individuals exposed to 
      several adverse environmental factors, the overall rate of numerical chromosome 
      aberrations in cultivated cells after at least one DNA replication cycle exceeded 
      that in noncultivated cells by 52% (P = 0.01), whereas only a trend for its 
      increase was observed in the control group (23%, P = 0.25). Thus, the effect of 
      adverse environmental factors in humans caused more than a twofold increase in 
      the difference between the rates of aneuploid cells in cultivated and 
      non-cultivated leukocytes in the exposed group as compared to control. It is 
      conjectured that cell division is accompanied by the expression of potential 
      damage of mitotic chromatid segregation apparatus accumulated in vivo. These 
      defects, realized during cell division, bring about numerical chromosome 
      aberrations.
FAU - Nazarenko, S A
AU  - Nazarenko SA
AD  - Research Institute of Medical Genetics, Tomsk Research Center, Russian Academy of 
      Medical Sciences, Tomsk, 634050 Russia. snaz@img.tsu.ru
FAU - Timoshevsky, V A
AU  - Timoshevsky VA
LA  - rus
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Genetika
JT  - Genetika
JID - 0047354
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Cells, Cultured
MH  - Chromosome Aberrations/*radiation effects
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Gamma Rays/*adverse effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - *Leukocytes
MH  - Male
MH  - Middle Aged
MH  - Occupational Exposure/adverse effects
EDAT- 2005/05/04 09:00
MHDA- 2005/06/17 09:00
CRDT- 2005/05/04 09:00
PHST- 2005/05/04 09:00 [pubmed]
PHST- 2005/06/17 09:00 [medline]
PHST- 2005/05/04 09:00 [entrez]
PST - ppublish
SO  - Genetika. 2005 Mar;41(3):391-5.