PMID- 15865974
OWN - NLM
STAT- MEDLINE
DCOM- 20050808
LR  - 20061115
IS  - 0076-6879 (Print)
IS  - 0076-6879 (Linking)
VI  - 395
DP  - 2005
TI  - In situ hybridization of phytoplankton using fluorescently labeled rRNA probes.
PG  - 299-310
AB  - Phytoplankton are one of the major components of ecosystem processes and play an 
      important role in many biogeochemical cycles in the marine and freshwater 
      environment. Despite their importance, many microalgae are poorly described and 
      little is known of broad spatial and temporal scale trends in their abundance and 
      distribution. Reasons for this are that microalgae are often small, lack distinct 
      morphological features, and are unculturable, which make analyses difficult. It 
      is now possible by using molecular biological techniques to advance our knowledge 
      of aquatic biodiversity and to understand how biodiversity supports ecosystem 
      structure, dynamics, and resilience. We present in this chapter a brief review of 
      the progress that has been made in analyzing microalgae from populations to the 
      species level. The described methods range from DNA fingerprinting techniques, 
      such as random amplified polymorphic DNA (RAPD), amplified fragment length 
      polymorphisms (AFLPs), and simple sequence repeats (SSRs), to microsatellites, 
      which are used in population studies, to sequence analysis, which help to 
      reconstruct the evolutionary history of organisms and to examine relationships at 
      various taxonomic levels. Special emphasis is given to the application of 
      molecular probes for the identification and characterization of microalgal taxa. 
      The fast and secure identification of phytoplankton, especially of toxic species, 
      is important from an ecological and economical point of view and whole-cell 
      hybridization with specific fluorochrome-labeled probes followed by fluorescence 
      microscopy or flow cytometry offers a fast method for this purpose. In this 
      context, we present a detailed protocol for fluorescence in situ hybridization 
      (FISH) of ribosomal RNA (rRNA) probes that can be applied to many algal cell 
      types and discuss practical considerations of its use.
FAU - Groben, Rene
AU  - Groben R
AD  - Alfred Wegener Institute, D-27570 Bremerhaven, Germany.
FAU - Medlin, Linda
AU  - Medlin L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Fixatives)
RN  - 0 (Indicators and Reagents)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Algal)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - DNA Fingerprinting
MH  - Ecosystem
MH  - Fixatives
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indicators and Reagents
MH  - Molecular Probe Techniques
MH  - Phytoplankton/*genetics/isolation & purification
MH  - RNA Probes
MH  - RNA, Algal/*genetics
MH  - RNA, Ribosomal/*genetics
MH  - Sequence Analysis, RNA
RF  - 34
EDAT- 2005/05/04 09:00
MHDA- 2005/08/09 09:00
CRDT- 2005/05/04 09:00
PHST- 2005/05/04 09:00 [pubmed]
PHST- 2005/08/09 09:00 [medline]
PHST- 2005/05/04 09:00 [entrez]
AID - S0076687905950180 [pii]
AID - 10.1016/S0076-6879(05)95018-0 [doi]
PST - ppublish
SO  - Methods Enzymol. 2005;395:299-310. doi: 10.1016/S0076-6879(05)95018-0.